Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10479-CD-050
Recombinant Human CD300e/LMIR6 Fc Chimera Protein Bioactivity
2 Images
Product Details
FAQs
Reviews

Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 0.6-6.0 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CD300e/LMIR6 protein
Human CD300e/LMIR6
(Leu18-Leu169)
Accession # Q496F6.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44 kDa
SDS-PAGE
45-58 kDa and 106-125 kDa (non-reducible dimer), under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10479-CD

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human CD300e/LMIR6 Fc Chimera Protein Bioactivity View Larger

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 0.6-6.0 μg/mL.

SDS-PAGE Recombinant Human CD300e/LMIR6 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD300e/LMIR6 Fc Chimera Protein (10479-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-58 kDa/106-125 kDa (non-reducible dimer) and 106-125 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD300e/LMIR6

CD300e, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35-kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 156 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 11 aa cytoplasmic stub (2). The ECD contains an Ig-like V-type domain with a single disulfide bond and an N-linked glycosylation site. Within the ECD, human CD300e shares 57% sequence identity with mouse and rat CD300e. Its transmembrane segment contains a charged lysine residue which mediates CD300e association with the signaling adaptor molecule DAP12 and enables CD300e to function as an activating receptor (2, 3, 5). The ligand of CD300e is unknown; sphingomyelin has been demonstrated to bind human and mouse CD300e (5). CD300e is highly expressed on intermediate and non-classical monocytes, and myeloid dendritic cells (3, 4). CD300e on monocytes from HIV-infected patients under cART is correlated with markers of disease progression and immune inflammation (6). The cross-linking of CD300e enhances LPS-mediated cytokine production by monocytes, enhancing TNF-alpha production while no enhancement is observed on IL-1 beta, IL-6 and IL-10 (7). Our in-house data indicate that CD300e inhibits T cell activation, including anti-CD3-induced IL-2 and IFN-gamma secretion.

References
  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.
  3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.
  4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.
  5. Isobe, M. et al. (2018) J. Biol. Chem. 293:3793.
  6. Vitalle, J. et al. (2017) Front. Immunol. 8:836.
  7. Zenarruzabeitia O. et al. (2016) Sci Rep. 6:32693.
Entrez Gene IDs
342510 (Human); 217306 (Mouse)
Alternate Names
CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review