Recombinant Human CD37 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10160-CD-050
Recombinant Human CD37 Fc Chimera Protein, CF SDS-PAGE
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Recombinant Human CD37 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD37 Fc Chimera (Catalog # 10160-CD) is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human CD19 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 5-25 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD37 protein
Human CD37
(Thr110-Asn241)
Accession # P11049
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Thr110
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42 kDa
SDS-PAGE
54-63 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10160-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD37 Fc Chimera (Catalog # 10160-CD) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing major bands at 54-63 kDa and 108-126 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD37

CD37, also known as TSPAN26, is a 40‑45 kDa palmitoylated traspanin superfamily glycoprotein that is mainly expressed by B cell lymphocytes and myeloid cells (1). Tetraspanins are characterized by four transmembrane domains, one small extracellular loop (SEL) and one large extracellular loop (LEL), and short N- and C-termini (1). The CD37-LEL has the most variation across these different domains, but a CCG motif and the position of the last cysteine residue in the LEL are conserved in all tetraspanins (2). Tetraspanins form complexes with a wide range of transmembrane and cytoplasmic proteins, including integrins, forming small networks referred to as tetraspanin enriched microdomains, or TEMs (1). Human CD37-LEL shares 66% amino acid sequence identity with mouse and rat CD37. CD37 directly associates with dectin-1 on macrophages and stabilizes cell surface dectin-1 expression (3). It also inhibits T cell activation and proliferation by limiting T cell receptor signaling (4). Mice deficient in CD37 develop B cell lymphoma (5). Kidneys of CD37-/- mice showed more mesangial proliferation, endothelial cell activation, podocyte activation, and segmental podocyte foot process effacement compared to the CD37/Il6 double-knockout mice, emphasizing that IL-6 mediates renal pathology in CD37-/- mice. Thus, CD37 may protect against IgA nephropathy by inhibition of the IL-6 pathway (6).

References
  1. Charrin, S. et al. (2002) FEBS Lett. 516:139.
  2. Berditchevski, Fedor. (2001) J. Cell. Sci. 114:4143.
  3. Meyer-Wentrup, F. et al. (2007) J Immunol. 178:154.
  4. van Spriel, A.B. et al. (2004) J Immunol 172:2953.
  5. de Winde, C.M. et al. (2016) J Clin Invest. 126:653.
  6. Rops, ALWMM et al. (2018) Kidney Int. 93:1356.
Entrez Gene IDs
951 (Human); 12493 (Mouse); 29185 (Rat)
Alternate Names
CD37 antigentetraspanin-26; CD37 molecule; CD37; cell differentiation antigen 37; GP52-40; leukocyte antigen CD37; MGC120234; Tetraspanin-26; TSPAN26; tspan-26; TSPAN26leukocyte surface antigen CD37

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