Recombinant Human CD37 Fc Chimera Protein, CF Summary
Accession # P11049
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human CD37 Fc Chimera (Catalog # 10160-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing major bands at 54-63 kDa and 108-126 kDa, respectively.
CD37, also known as TSPAN26, is a 40‑45 kDa palmitoylated traspanin superfamily glycoprotein that is mainly expressed by B cell lymphocytes and myeloid cells (1). Tetraspanins are characterized by four transmembrane domains, one small extracellular loop (SEL) and one large extracellular loop (LEL), and short N- and C-termini (1). The CD37-LEL has the most variation across these different domains, but a CCG motif and the position of the last cysteine residue in the LEL are conserved in all tetraspanins (2). Tetraspanins form complexes with a wide range of transmembrane and cytoplasmic proteins, including integrins, forming small networks referred to as tetraspanin enriched microdomains, or TEMs (1). Human CD37-LEL shares 66% amino acid sequence identity with mouse and rat CD37. CD37 directly associates with dectin-1 on macrophages and stabilizes cell surface dectin-1 expression (3). It also inhibits T cell activation and proliferation by limiting T cell receptor signaling (4). Mice deficient in CD37 develop B cell lymphoma (5). Kidneys of CD37-/- mice showed more mesangial proliferation, endothelial cell activation, podocyte activation, and segmental podocyte foot process effacement compared to the CD37/Il6 double-knockout mice, emphasizing that IL-6 mediates renal pathology in CD37-/- mice. Thus, CD37 may protect against IgA nephropathy by inhibition of the IL-6 pathway (6).
- Charrin, S. et al. (2002) FEBS Lett. 516:139.
- Berditchevski, Fedor. (2001) J. Cell. Sci. 114:4143.
- Meyer-Wentrup, F. et al. (2007) J Immunol. 178:154.
- van Spriel, A.B. et al. (2004) J Immunol 172:2953.
- de Winde, C.M. et al. (2016) J Clin Invest. 126:653.
- Rops, ALWMM et al. (2018) Kidney Int. 93:1356.
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