Recombinant Human CD52 Fc Chimera Protein, CF

R&D Systems | Catalog # 9116-CD

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human CD52 Fc Chimera Protein (9116-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P31358

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD52 protein
Human CD52
(Gly25-Ser36)
Accession # P31358		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly25

Predicted Molecular Mass

28 kDa

SDS-PAGE

38 - 44 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD52 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human
Siglec‑10 Fc Chimera (Catalog # 2130-SL) that produces 50% of the optimal binding response is approximately 1-5 μg/mL.

Scientific Data Images for Recombinant Human CD52 Fc Chimera Protein, CF

Recombinant Human CD52 Fc Chimera Protein Bioactivity

Recombinant Human CD52 Fc Chimera Protein Bioactivity

When Recombinant Human CD52 Fc Chimera is coated at 1 µg/mL, Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) binds with a typical ED50 of 1-5 µg/mL.

Formulation, Preparation, and Storage

9116-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD52

CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis (1, 2). Mature human CD52 is a 12 amino acid peptide that is tethered to the cell surface with a GPI linkage (3). It carries a large tissue-specific carbohydrate structure which increases the molecular weight of CD52 to 15-20 kDa (3-5). CD52 is expressed on lymphocytes, monocytes, monocycte-derived dendritic cells, eosinophils, and neutrophils (6-8), as well as on mature spermatozoa and epithelial cells lining the male genital tract (5, 9, 10). It protects sperm against anti-sperm antibodies by binding to C1q and inhibiting complement activation (11). CD52 ligation on CD4+ T cells induces a suppressive population of cells that release soluble CD52 which then binds to Siglec-10 (12-14). This interaction inhibits the proliferation of activated T cells and the cytotoxic function of autoimmune CD8+ T cells in type 1 diabetes (13).

References

  1. Morris, P.J. and N.K. Russell (2006) Transplantation 81:1361.
  2. Redpath, S. et al. (1998) Immunology 93:595.
  3. Xia, M.Q. et al. (1991) Eur. J. Immunol. 21:1677.
  4. Treumann, A. et al. (1995) J. Biol. Chem. 270:6088.
  5. Schroter, S. et al. (1999) J. Biol. Chem. 274:29862.
  6. Ratzinger, G. et al. (2003) Blood 101:1422.
  7. Elsner, J. et al. (1996) Blood 88:4684.
  8. Ambrose, L.R. et al. (2009) Blood 114:3052.
  9. Focarelli, R et al. (1998) Mol. Hum. Reprod. 4:119.
  10. Hale, G. et al. (1993) J. Reprod. Immunol. 23:189.
  11. Hardiyanto, L. et al. (2012) J. Reprod. Immunol. 94:142.
  12. Watanabe, T. et al. (2006) Clin. Immunol. 120:247.
  13. Bandala-Sanchez, E. et al. (2013) Nat. Immunol. 14:741.
  14. Toh, B.-H. et al. (2013) Cell. Mol. Immunol. 10:379.

Alternate Names

CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP

Entrez Gene IDs

1043 (Human); 23833 (Mouse)

Gene Symbol

CD52

UniProt

P31358

Additional CD52 Products

Product Documents for Recombinant Human CD52 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD52 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human CD52 Fc Chimera Protein, CF

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