Recombinant Human Siglec-10 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2130-SL-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Human Siglec-10 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated 6’-Sialyllactose-Polyacrylamide in functional ELISA. Whitney, G. et al. (2001) FEBS 268:6083. rhSiglec-10/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody (Catalog # G-102-C) coated plate can bind biotinylated 6’-Sialyllactose-Polyacrylamide with a linear range of 0.04-2.5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Siglec-10 protein
Human Siglec-10
(Met17-Thr546)
Accession # Q96LC7
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met17
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
85 kDa (monomer)
SDS-PAGE
105-110 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2130-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Siglec-10

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1-3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V-type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5-8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5-7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is upregulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell.(7) When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

References
  1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
  5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
  6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
  7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
  8. Munday, J. et al. (2001) Biochem. J. 355:489.
  9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
  10. Aizawa, H. et al. (2003) Genomics 82:521.
  11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
Long Name
Sialic Acid Binding Ig-like Lectin 10
Entrez Gene IDs
89790 (Human)
Alternate Names
MGC126774; PRO940; sialic acid binding Ig-like lectin 10; sialic acid-binding Ig-like lectin 10; Siglec10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Citations for Recombinant Human Siglec-10 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
    Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
    PLoS ONE, 2019;14(7):e0219480.
    Species: Human
    Sample Types: Natural Protein
    Applications: Bioassay
  2. Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
    Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
    Elife, 2014;3(0):e04066.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  3. Amelioration of sepsis by inhibiting sialidase-mediated disruption of the CD24-SiglecG interaction.
    Authors: Chen GY, Chen X, King S
    Nat. Biotechnol., 2011;29(5):428-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay

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Recombinant Human Siglec-10 Fc Chimera Protein, CF
By Anonymous on 10/17/2019
Application: In vivo study