Recombinant Human CD63 Fc Chimera Protein, CF Summary
Accession # P08962-1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human CD63 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 41-60 kDa and 80-120 kDa, respectively.
CD63, also known as Tspan-30, is a member of the tetraspanin family of proteins that mediate signal transduction events which play a role in the regulation of cell development, activation, growth and motility (1, 2). These membrane proteins are characterized by the presence of four transmembrane domains, a small extracellular loop (SEL) and a large extracellular loop (LEL) (2, 3). Regions of the tetraspanin LEL are known to associate with integrins to regulate membrane protein trafficking, leukocyte recruitment, and adhesion processes (4, 5). Deficiency of CD63 is associated with Hermansky-Pudlak syndrome (6). Human CD63-LEL shares 67% and 66% aa sequence identity with mouse and rat CD63-LEL, respectively. CD63 has been identified as a cell surface binding partner for tissue inhibitor of metalloproteinase 1 (TIMP-1), modulating the tetraspanin/integrin signaling complex which regulates cell survival and polarization (7).
- Kang, M. et al. (2014) PLOS One. 9:e102817.
- Yu, M. et al. (2016) J. Immunol. Res. 2016:1.
- Chaiyadet, S. et al. (2017) Sci. Rep 7:14342.
- Tugues, S. et al. (2013) J. Biol. Chem. 288:19060.
- Yañez-Mo, M. et al. (2009) Trends Cell Biol. 19:434.
- Nishibori, M. et al. (1993) J. Clin. Invest. 91:1775.
- Jung, K.K. et al. (2006) EMBO J. 25:3934.
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