Recombinant Human CD63 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5048-CD-050
Recombinant Human CD63 Fc Chimera Protein, CF SDS-PAGE
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Recombinant Human CD63 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant CD63 Fc Chimera is used at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human TIMP-1 that produces 50% optimal binding response is 3-15 μg/mL.   
Source
Chinese Hamster Ovary cell line, CHO-derived human CD63 protein
MD
Human IgG1
(Pro100-Lys330)
IEGR Human CD63
(Ala103-Val203)
Accession # P08962-1
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
38 kDa
SDS-PAGE
41-60 kDa, reducing conditions

Product Datasheets

Data Image

SDS-PAGE Recombinant Human CD63 Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD63 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 41-60 kDa and 80-120 kDa, respectively.

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Background: CD63

CD63, also known as Tspan-30, is a member of the tetraspanin family of proteins that mediate signal transduction events which play a role in the regulation of cell development, activation, growth and motility (1, 2). These membrane proteins are characterized by the presence of four transmembrane domains, a small extracellular loop (SEL) and a large extracellular loop (LEL) (2, 3). Regions of the tetraspanin LEL are known to associate with integrins to regulate membrane protein trafficking, leukocyte recruitment, and adhesion processes (4, 5). Deficiency of CD63 is associated with Hermansky-Pudlak syndrome (6). Human CD63-LEL shares 67% and 66% aa sequence identity with mouse and rat CD63-LEL, respectively.  CD63 has been identified as a cell surface binding partner for tissue inhibitor of metalloproteinase 1 (TIMP-1), modulating the tetraspanin/integrin signaling complex which regulates cell survival and polarization (7).

References
  1. Kang, M. et al. (2014) PLOS One. 9:e102817.
  2. Yu, M. et al. (2016) J. Immunol. Res. 2016:1.
  3. Chaiyadet, S. et al. (2017) Sci. Rep 7:14342.
  4. Tugues, S. et al. (2013) J. Biol. Chem. 288:19060.
  5. Yañez-Mo, M. et al. (2009) Trends Cell Biol. 19:434.
  6. Nishibori, M. et al. (1993) J. Clin. Invest. 91:1775.
  7. Jung, K.K. et al. (2006) EMBO J. 25:3934.

Entrez Gene IDs
967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
Alternate Names
CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1lysosome-associated membrane glycoprotein 3; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30; TSPAN30granulophysin

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Primary Antibodies

Human IgG1 Antibody

Human IgG Fc Antibody

WB,Flow,CyTOF-ready

Human IgG1 Antibody

Recombinant Proteins

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