Recombinant Human CD72 Fc Chimera Protein, CF

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Recombinant Human CD72 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to induce IFN-gamma secretion in NK‑92 human natural killer lymphoma cells. The ED50 for this effect is 3-15 μg/mL.
Mouse myeloma cell line, NS0-derived human CD72 protein
Human CD72
Accession # P21854
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
54.6 kDa (monomer)
60-65 kDa, reducing conditions

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Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD72

CD72, also known as Lyb‑2, is a 40‑45 kDa type II transmembrane glycoprotein that plays a role in immune system regulation (1). Mature human CD72 consists of a 95 amino acid (aa) cytoplasmic domain with two immunoreceptor tyrosine‑based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain with a coiled‑coil domain and a C‑type lectin domain (2). Within the ECD, human CD72 shares 48% and 44% aa sequence identity with mouse and rat CD72, respectively. CD72 is expressed on B lineage cells, NK cells, monocytes, dendritic cells, and mast cells (2‑6). CD72 binds to CD5 with mouse/human cross‑reactivity and to Semaphorin 4D/CD100 (5, 7‑9). It associates with CD79A in the B cell antigen receptor (BCR) complex following antigen stimulation and dampens BCR signaling through interactions with the phosphatase SHP‑1 (10). CD72 ligation with antibodies or with Semaphorin 4D induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP‑1, leading to B cell proliferation (5, 9). Both CD72 and Semaphorin 4D are required for the maintenance of B cell anergy and the regulation of peripheral B cell tolerance as shown by the development of autoimmunity in mice that lack either molecule (10, 11). In addition to its negative regulation of BCR signaling, CD72 can induce positive signaling in B cells independent of the BCR (12). CD72 binding to Semaphorin 4D induces cytokine production by monocytes and dendritic cells, inhibits SCF R/c‑kit induced mast cell proliferation and activation, and inhibits the cytolytic activity of NK cells (4‑6). Semaphorin 4D is expressed on activated NK cells and contributes to the adhesive interaction between NK and CD72+ target cells leading to a more efficent killing and enhanced IFN-gamma secretion (13).

  1. Nykimbeng-Takwi, E. and S.P. Chapoval (2011) Immunol. Res. 50:10.
  2. Von Hoegen, I. et al. (1990) J. Immunol. 144:4870.
  3. Nakayama, E. et al. (1989) Proc. Natl. Acad. Sci. 86:1352.
  4. Alcon, V.L. et al. (2009) Eur. J. Immunol. 39:826.
  5. Ishida, I. et al. (2003) Int. Immunol. 15:1027.
  6. Kataoka, T.R. et al. (2010) J. Immunol. 184:2468.
  7. Van de Velde, H. et al. (1991) Nature 351:662.
  8. Luo, W. et al. (1992) J. Immunol. 148:1630.
  9. Kumanogoh, A. et al. (2000) Immunity 13:621.
  10. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
  11. Li, D. H.-H. et al. (2008) Arthritis Rheum. 58:3192.
  12. Wu, H.-J. et al. (2001) J. Immunol. 167:1263.
  13. Mizrahi, S. et al. (2007) PLoS ONE 9:e818.
Entrez Gene IDs
971 (Human); 12517 (Mouse)
Alternate Names
CD72 antigenB-cell differentiation antigen CD72; CD72 molecule; CD72; CD72b; Ly-19.2; Ly-32.2; Lyb-2; LYB2lyb-2


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