Recombinant Human CD94 Fc Chimera Protein, CF Summary
Accession # Q13241.2
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in PBS with Trehalose.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human NKG2E Fc Chimera (9759-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human CD94 Fc Chimera (Catalog # 10281-CD) binds with an ED50 of 1.25-10 µg/mL.
2 μg/lane of Recombinant Human CD94 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-62 kDa.
CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).
- Chester, C. et al. (2015) Front. Immunol. 6:601.
- Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
- Lieto, L.D. et al. (2006) Genes Immun. 7:36.
- Yu, J. et al. (2010) Blood 115:274.
- Phillips, J.H. et al. (1996) Immunity 5:163.
- Lazetic, S. et al. (1996) J. Immunol. 157:4741.
- Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
- Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
- Braud, V.M. et al. (1998) Nature 391:795.
- Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
- Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
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