Recombinant Human NKG2E Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9759-NK-050
Recombinant Human NKG2E Fc Chimera Protein Binding Activity
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Recombinant Human NKG2E Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD94 (Catalog # 9270-CD) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human NKG2E binds with an ED50 of 1-7 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human NKG2E protein
MDHuman IgG1
(Pro100-Lys330)
IEGRHuman NKG2E
(Glu98-Ser240)
Accession # Q07444-1
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43 kDa
SDS-PAGE
52-63 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9759-NK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human NKG2E Fc Chimera Protein Binding Activity View Larger

When Recombinant Human CD94 (Catalog # 9270-CD) is coated at 1 µg/mL, Recombinant Human NKG2E Fc Chimera (Catalog # 9759-NK) binds with an ED50 of 1-7 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKG2E

Human NKG2E (NK cell Group 2 isoform E; Killer cell lectin-like receptor subfamily C, member 3) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). Human NKG2E consist of a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 147 aa extracellular domain (ECD). Within the ECD, human NKG2E shares 40% sequence identity with mouse NKG2E. NKG2E-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (7, 8). Triggering the NKG2E-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (1, 7, 9). Over-expression of KLRC3/NKG2E in glioblastomas may play a major role in glioblastoma aggressiveness and recurrence (10).

References
  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi, I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  8. Kaiser, B.K. et al. (2005) J Immunol 174:2878.
  9. Bellón, T. et al. (1999) J Immunol 162:3996.
  10. Cheray, M. et al. (2017) J Cell Mol Med 21:244.
Long Name
Natural Killer G2E
Entrez Gene IDs
3823 (Human); 58179 (Mouse)
Alternate Names
killer cell lectin-like receptor subfamily C, member 3; NK cell receptor E; NKG2-E type II integral membrane protein; NKG2E; NKG2-E-activating NK receptor; NKG2ENKG2-E

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