Recombinant Human CD99 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
3968-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (5)
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Recombinant Human CD99 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated rhCD99 in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived human CD99 protein
Human CD99
(Asp23 - Asp122)
Accession # P14209
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
36.7 kDa (monomer)
SDS-PAGE
40-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3968-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD99

CD99 (also named MIC2, E2 and thymic leukemia antigen) is the founding member of the CD99 family of molecules. The CD99 family contains four members; CD99, CD99L2, XG and the pseudogene CD99L1 (1, 2, 3). Native human CD99 is 32 kDa in size and exists as a type I transmembrane glycoprotein. This is referred to as the long, or type I isoform. It is synthesized as a 185 amino acid (aa) precursor that contains a 22 aa signal sequence, a 100 aa extracellular domain (ECD), a 25 aa transmembrane segment, and a 38 aa cytoplasmic region (4). The ECD contains no identifiable motifs, N-linked glycosylation sites, or cysteine residues; it does possess sites for O-linked glycosylation. The cytoplasmic region, albeit short, does have signal transduction capability (5). There are apparently multiple isoforms for human CD99. One shows a 16 aa deletion in the ECD (aa 34 - 49), a second shows a 38 aa deletion in the cytoplasmic region (aa 122 - 159), and a third exhibits a three aa truncation at the C-terminus (6, 7, 8). The best studied isoform shows an Asp-Gly substitution for the C-terminal 27 amino acids. This is referred to as the 28 kDa type II isoform (9). The type I and II isoforms have distinctive signal transduction pathways (FAK-src for type I; PI3K plus src-ERK1/2 for type II), and mediate clearly different biological outcomes (5, 9, 10). The two numbered isoforms may or may not coexist on the same cells. Peripheral T cells have only the long isoform, while double-positive thymocytes express both isotypes. What is unclear is the monomeric vs. dimeric status of CD99. In mouse, CD99 reportedly forms disulfide-linked homodimers (11). In human, however, CD99 is reportedly monomeric if only a type I isoform, and a covalent heterodimer if coexpressing type I and II isoforms (12, 13). Cells known to express CD99 include fibroblasts, neutrophils, T cells, double-positive thymocytes, CD34+ stem cells, monocytes and endothelial cells (2, 12, 14, 15). Homophilic interaction between CD99 on the neutrophil and CD99 on the endothelial cell regulates the transendothelial migration of neutrophils during inflammation (16). Human CD99 is only 48% aa identical to mouse CD99 (17).

References
  1. Wilson, M.D. et al. (2006) Physiol Genomics 27:201. 
  2. Petri, B. and M.G. Bixel (2006) FEBS J. 273:4399. 
  3. Suh, Y.H. et al. (2003) Gene 307:63. 
  4. Gelin, C. et al. (1989) EMBO J. 8:3253. 
  5. Byun, H-J. et al. (2006) J. Biol. Chem. 281:34833. 
  6. GenBank Accession # EAW98698. 
  7. GenBank Accession # EAW98699. 
  8. GenBank Accession # EAW98700. 
  9. Hahn, H-J. et al. (1997) J. Immunol. 159:2250.
  10. Scotlandi, K. et al. (2007) Oncogene Apr 30; [Epub ahead of print].
  11. Park, S.H. et al. (2005) Gene 353:177.
  12. Schenkel, A.R. et al. (2002) Nat. Immunol. 3:143.
  13. Alberti, I. et al. (2002) FASEB J. 16:1946.
  14. Imbert, A-M. et al. (2006) Blood 108:2578.
  15. Dworzak, M.N. et al. (1994) Blood 83:415.
  16. Lou, O. et al. (2007) J. Immunol. 178:1136
  17. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
Entrez Gene IDs
4267 (Human); 673094 (Mouse); 652929 (Rat)
Alternate Names
12E7; CD99 antigenY homolog; CD99 molecule; CD99; E2 antigen; HBA71; MIC2 (monoclonal 12E7); MIC2; MIC2Y; MSK5X; pilr-1; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2

Citations for Recombinant Human CD99 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. GDF6-CD99 Signaling Regulates Src and Ewing Sarcoma Growth
    Authors: F Zhou, DJ Elzi, P Jayabal, X Ma, YC Chiu, Y Chen, B Blackman, ST Weintraub, PJ Houghton, Y Shiio
    Cell Rep, 2020;33(5):108332.
    Species: Human
    Sample Types: Protein
    Applications: Functional Assay
  2. Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin ? and promotes transendothelial cell migration
    Authors: Tillmann Bedau
    FASEB J, 2016;0(0):.
    Applications: ELISA (Standard)
  3. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
    Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
    PLoS ONE, 2014;9(9):e107920.
    Species: Bacteria
    Sample Types: Protein
    Applications: Enzyme Assay Substrate
  4. Metalloprotease-mediated tumor cell shedding of B7-H6, the ligand of the natural killer cell-activating receptor NKp30.
    Authors: Schlecker E, Fiegler N, Arnold A, Altevogt P, Rose-John S, Moldenhauer G, Sucker A, Paschen A, von Strandmann E, Textor S, Cerwenka A
    Cancer Res, 2014;74(13):3429-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  5. Human cytomegalovirus infection elicits new decidual natural killer cell effector functions.
    Authors: Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N
    PLoS Pathog, 2013;9(4):e1003257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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