Recombinant Human CEACAM-20 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.5-2.5 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human CEACAM-20
    (Gln31-Gly450)
    Accession # Q6UY09
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Leu32
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    73 kDa
  • SDS-PAGE
    82-105 kDa, reducing conditions
9427-CM
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Immobilized Recombinant Human CEACAM-20 Fc Chimera (Catalog #9427-CM) enhances L Cells mouse fibroblasts adhesion. The ED50 for this effectis 0.5-2.5 μg/mL.
Background: CEACAM-20
Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-20 consists of a 420 aa extracellular domain, a 21 aa helical transmembrane, and a 114 aa cytoplasmic domain. The extracellular domain possesses four IgC2-like domains, which are stabilized by disulfide bonds, as well as several predicted glycosylation sites (1-5). The extracellular domain of CEACAM-20 is also unique among the CEACAMs because it contains a truncated IgV-like N domain (2). The extracellular domain of human CEACAM-20 has 64% and 62% aa identity to the mouse and rat protein, respectively. Also, the cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). Human CEACAM proteins have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, and apoptosis (7, 8). Human CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought be act as a physiological substrate for SAP-1 in the intestinal epithelium (10).
  • References:
    1. Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
    2. Zhang, H. et al. (2013) PLoS ONE 8:e53359.
    3. Zebhauser, R. et al. (2005) Genomics 86:566.
    4. Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
    5. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
    6. Murata, Y. et al. (2015) PNAS E4264-4271.
    7. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
    8. Horst, AK. Wagener, C. (2004) Handb Exp Pharmacol 283-341.
    9. Kitamura, Y. et al. (2015) Genes to Cells 20:578.
    10. Kotani, et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.
  • Long Name:
    Carcinoembryonic Antigen-related Cell Adhesion Molecule 20
  • Entrez Gene IDs:
    125931 (Human)
  • Alternate Names:
    CEACAM20; CEACAM-20; GPAD9366; UNQ9366
Related Research Areas

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