Recombinant Human CEACAM-20 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10277-CM-050
Recombinant Human CEACAM-20 His-tag Protein, CF Bioactivity
1 Image
Product Details
FAQs
Reviews

Recombinant Human CEACAM-20 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.25-1.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CEACAM-20 protein
Gln31-Gly450, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln31 inferred from enzymatic pyroglutamate treatment revealing Leu32
Predicted Molecular Mass
47 kDa
SDS-PAGE
73-84 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10277-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Immobilized Recombinant Human CEACAM-20 His-tag (Catalog # 10277-CM) enhances L cells mouse fibroblast cell line adhesion. The ED50for this effect is 0.25‑1.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CEACAM-20

Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-20 consists of a 420 amino acid (aa) extracellular domain (ECD), a 21 aa helical transmembrane segment, and a 114 aa cytoplasmic domain. The extracellular domain possesses four IgC2-like domains which are stabilized by disulfide bonds, as well as several predicted glycosylation sites (1-5). The extracellular domain of CEACAM-20 is also unique among the CEACAMs because it contains a truncated IgV-like N domain (2). Within the ECD, human CEACAM-20 shares 64% and 62% aa identity with the mouse and rat CEACAM-20, respectively. The cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). Human CEACAM proteins have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, and apoptosis (7, 8). Human CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought to act as a physiological substrate for SAP-1 in the intestinal epithelium (10).

References
  1. Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
  2. Zhang, H. et al. (2013) PLoS ONE 8:e53359.
  3. Zebhauser, R. et al. (2005) Genomics 86:566.
  4. Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
  5. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
  6. Murata, Y. et al. (2015) PNAS E4264.
  7. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  8. Horst, AK. Wagener, C. (2004) Handb Exp Pharmacol 283.
  9. Kitamura, Y. et al. (2015) Genes to Cells 20:578.
  10. Kotani,  T. et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 20
Entrez Gene IDs
125931 (Human)
Alternate Names
CEACAM20; CEACAM-20; GPAD9366; UNQ9366

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human CEACAM-20 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CEACAM-20 His-tag Protein, CF and earn rewards!

Have you used Recombinant Human CEACAM-20 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review