Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9985-CM-050
Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF Binding Activity
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Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM‑3/CD66d Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED50 of 0.05-0.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CEACAM-3/CD66d protein
Human CEACAM-3
(Lys35-Gly155)
Accession # P40198
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Lys35
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
39.7 kDa
SDS-PAGE
48-54 kDa, reducing conditions

Product Datasheets

9985-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF Binding Activity View Larger

When Recombinant Human CEACAM-3/CD66d Fc Chimera (Catalog #9985-CM) is immobilized at 1 µg/mL, Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED50 of 0.05‑0.4 μg/mL.

SDS-PAGE Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human CEACAM‑3 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 48-54 kDa and 96-110 kDa, respectively.

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Background: CEACAM-3/CD66d

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3 (CEACAM-3), or CD66d, is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Human CEACAM-3 is ~35 kDa, consisting of an extracellular domain (ECD) containing one IgV-like domain, a single transmembrane domain and a cytoplasmic tail. The cytoplasmic tail of CEACAM-3 contains an immunoreceptor tyrosine-based activation motif (ITAM), which recruits kinases to propagate pro-inflammatory signaling cascades (3). Interestingly, CEACAM-3 appears to be primate specific, with on non-primate orthologs currently identified (4). Originally discovered as a biomarker for colorectal cancer (5), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (6-8). Unlike other CEA family members, CEACAM-3 has not been shown to form cell–cell adhesion interactions with other CEACAM family members (9). CEACAM-3 has been found to be specifically expressed on human neutrophils and other granulocytes and appears to be a specific adaptation of the innate immune system to cope with a small set of host-specific pathogen (9). CEACAM-3 was identified as critical for opsonin-independent phagocytosis and bacterial clearance (10). CEACAM-3 binds to the colony opacity-associated (Opa) outer membrane proteins of bacteria, such as Neisseria gonorrhoeae, and triggers uptake of the pathogen and subsequent elimination (9, 10).

References
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser R, et al. (2005) Genomics 86:566.
  3. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.
  4. Pavlopoulou A. and Scorilas A. (2014) Genome Biol Evol. 6:1314.
  5. Gold P and Freedman, S.O. (1965) J Exp Med. 122:467.
  6. Obrink, B. (1997) Curr Opin Cell Biol. 9:616.
  7. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol. 165:283.
  8. Kuespert K et al. (2006) Curr Opin Cell Biol. 18:565.
  9. Pils S. et al. (2008) Int J Med Microbiol. 298:553.
  10. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 3
Entrez Gene IDs
1084 (Human); 384557 (Mouse); 24926 (Rat)
Alternate Names
CD66d; CEA; CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3; CEACAM-3; CGM1; W264; W282

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