Recombinant Human CHIP/STUB1 Protein, CF

Catalog # Availability Size / Price Qty
E3-220-050
Product Details
Citations (1)
FAQs
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Recombinant Human CHIP/STUB1 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human CHIP/Stub1 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human CHIP/Stub1 concentration of 0.1-5 μM.
Source
E. coli-derived human CHIP/STUB1 protein
Contains a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
36 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-220

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% Glycerol (v/v), 5 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: CHIP/STUB1

STIP1 Homology and U-box Containing Protein 1 (STUB1), also known as Carboxyl Terminus of Hsp70-interacting Protein (CHIP), is a cytoplasmic protein that functions as a U-box Ubiquitin ligase (E3) (1). It is highly expressed in striated muscle and brain and has been observed at lower levels in other organs including the pancreas, lung, liver, and kidney (2,3). STUB1/CHIP is 303 amino acids (aa) in length with a predicted molecular weight of 34.8 kDa (2). Human STUB1/CHIP shares 97% aa sequence identity with the mouse ortholog but only 67% sequence identity with the rat ortholog (2). It consists of three 34 aa N-terminal tetratricopeptide repeat (TRP) domains (aa 26-127) that are responsible for protein-protein interactions and a C-terminal U-box domain (aa 226-300) that participates in ubiquitination (1,4,5). A central domain containing charged residues lies between the TRP and U-box domains and is thought to be necessary for TRP-dependent interactions (3). STUB1/CHIP participates in intracellular protein folding/refolding and degradation. It complexes with several molecular chaperone proteins, including HSP70/HSPA1A, HSC70, and HSP90, and modulates their activity (2,3,5,6). It also facilitates the ubiquitination of chaperone substrates, including nascent CFTR, phosphorylated Tau, p53, PTEN, Synuclein-alpha, and beta -APP, promoting their degradation (5,7-11). This recombinant human protein contains a C-terminal His6 tag.

References
  1. Jiang, J. et al. (2001) J. Biol. Chem. 276:42938.
  2. Ballinger, C.A. et al. (1999) Mol. Cell. Biol. 19:4535.
  3. McDonough, H. & C. Patterson (2003) Cell Stress Chaperones 8:303.
  4. Schulman, B.A. & Z.J. Chen (2005) Mol. Cell 20:653.
  5. Kumar, P. et al. (2012) J. Neurochem. 120:350.
  6. Connell, P. et al. (2001) Nat. Cell Biol. 3:93.
  7. Meacham, G.C. et al. (2001) Nat. Cell Biol. 3:100.
  8. Shin, Y. et al. (2005) J. Biol. Chem. 280:23727.
  9. Esser, C. et al. (2005) J. Biol. Chem. 280:27443.
  10. Kumar, P. et al. (2007) Hum. Mol. Genet. 16:848.
  11. Ahmed, S.F. et al. (2012) J. Biol. Chem. 287:15996.
Long Name
C-terminus of Hsc70 Interacting Protein
Entrez Gene IDs
10273 (Human); 56424 (Mouse)
Alternate Names
Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CHIPSTIP1 homology and U box-containing protein 1; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; EC 6.3.2.-; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STUB1; UBOX1

Citation for Recombinant Human CHIP/STUB1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Syntaphilin Ubiquitination Regulates Mitochondrial Dynamics And Tumor Cell Movements
    Authors: JH Seo, E Agarwal, KG Bryant, MC Caino, ET Kim, AV Kossenkov, HY Tang, LR Languino, DI Gabrilovic, AR Cohen, DW Speicher, DC Altieri
    Cancer Res., 2018;0(0):.
    Applications: Bioassay

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