Recombinant Human Chitinase 3-like 1 Protein, CF

Catalog # Availability Size / Price Qty
2599-CH-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Recombinant Human Chitinase 3-like 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 104 cells per well are added to recombinant human CHI3-L1 coated plate, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.6-2.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Chitinase 3-like 1 protein
Tyr22-Thr383, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Tyr22
Predicted Molecular Mass
41.3 kDa (monomer)
SDS-PAGE
38-41 kDa, reducing conditions

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2599-CH

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2599-CH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Chitinase 3-like 1

Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).

References
  1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
  2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
  4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
  5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
  6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
  7. Shao, R. et al. (2009) Oncogene 28:4456.
  8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
  9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
  10. Letuve, S. et al. (2008) J Immunol. 181:5167.
  11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
  12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
  13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
  14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
  15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
Entrez Gene IDs
1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)
Alternate Names
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

Citations for Recombinant Human Chitinase 3-like 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation
    Authors: SC Starossom, J Campo Garc, T Woelfle, S Romero-Sua, M Olah, F Watanabe, L Cao, A Yeste, JJ Tukker, FJ Quintana, J Imitola, F Witzel, D Schmitz, M Morkel, F Paul, C Infante-Du, SJ Khoury
    Nat Commun, 2019;10(1):217.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress.
    Authors: Park JA, Drazen JM, Tschumperlin DJ
    J. Biol. Chem., 2010;285(39):29817-25.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot

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Reviews for Recombinant Human Chitinase 3-like 1 Protein, CF

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Recombinant Human Chitinase 3-like 1 Protein, CF
By Anonymous on 11/06/2018
Application: Immunoassay Standard

The recombinant Chitinase 3-like 1 (YKL-40) worked well as a standard in an ELISA made to measure YKL-40 in human serum samples.


Recombinant Human Chitinase 3-like 1 Protein, CF
By Anonymous on 09/05/2018
Application: LCMS Biomarker discovery

We use this item as a positive control for IP experiments.