Recombinant Human Chitinase 3-like 2 Protein, CF

Catalog # Availability Size / Price Qty
5112-CH-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Chitinase 3-like 2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells.

When 5 x 104 cells per well are added to rhCHI3L2 coated plates for 30 minutes at 37 °C, cell adhesion is enhanced in a dose dependent manner with typical ED50 range of 0.7-2.8 μg/mL.

Opitimal dilutions should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived human Chitinase 3-like 2 protein
Tyr27-Leu390, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Tyr27
Predicted Molecular Mass
42.2 kDa
SDS-PAGE
40 kDa, reducing conditions

Product Datasheets

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5112-CH

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5112-CH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Chitinase 3-like 2

Chitinase 3-like 2 (CHI3L2), also called chondrocyte protein 39 or YKL-39, is a 39 kDa potentially glycosylated member of the glycosyl hydrolase 18 family (1 - 3). It is secreted by synovial fibroblasts, chondrocytes, and TGF beta /IL-4-stimulated monocyte-derived macrophages (1, 4, 5). The human CHI3L2 cDNA encodes 390 amino acids (aa), including a 26 aa signal sequence and a 364 aa mature region. Potential alternate start sites at Met-24 and Met+80 would create isoforms of 413 aa and 311 aa, while a potential deletion of aa 15 - 24 would create a 380 aa isoform (6). Mature human CHI3L2 shares 89%, 87% and 84% aa sequence identity with bovine, porcine and equine CHI3L2, respectively. The rodent genome does not include a CHI3L2 ortholog (2). Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1 - 3). Unlike CHI3L1, CHI3L2 does not bind heparin (1). While CHI3L1 is the major protein excreted by chondrocytes, up-regulation of CHI3L2 and not CHI3L1 correlates with osteoarthritic cartilage degeneration (1, 7, 8). CHI3L2 has been proposed as a potential biomarker for osteoarthritis (4, 7, 8). CHI3L1 has been found to coordinate adhesion receptors and promote cell signaling and tumor angiogenesis; like CHI3L1, CHI3L2 can enhance cell adhesion (9).

References
  1. Hu, B. et al. (1996) J. Biol. Chem. 271:19415.
  2. Bussink, A.P. et al. (2007) Genetics 177:959.
  3. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  4. Gratchev, A. et al. (2008) Biomarker Insights 3:39.
  5. Benz, K. et al. (2002) Biochem. Biophys. Res. Commun. 293:284.
  6. Entrez Accession # EAW56480, NP_001020370, NP_001020368.
  7. Steck, E. et al. (2002) Biochem. Biophys. Res. Commun. 299:109.
  8. Knorr, T. et al. (2003) Ann. Rheum. Dis. 62:995.
  9. Shao, R. et al. (2009) Oncogene 28:4456.
Entrez Gene IDs
1117 (Human)
Alternate Names
CHI3L2; Chitinase 3 like 2; Chitinase 3-like 2; chitinase-3-like protein 2; Chondrocyte protein 39; YKL39; YKL-39YKL39

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By Do-Hyun Kim on 12/01/2021
Application: Immunoassay Standard