Recombinant Human Chondroadherin His-tag Protein, CF

Catalog # Availability Size / Price Qty
8218-CH-050
Recombinant Human Chondroadherin His-tag Protein, CF Bioactivity
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Recombinant Human Chondroadherin His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4-2.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Chondroadherin protein
Ala22-Thr350, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala22 & Cys23
Predicted Molecular Mass
38 kDa
SDS-PAGE
33-38 kDa, reducing conditions

Product Datasheets

8218-CH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human Chondroadherin His-tag Protein, CF Bioactivity View Larger

Recombinant Human Chondroadherin (Catalog # 8218-CH) inducesadhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4-2.4 μg/mL.

SDS-PAGE Recombinant Human Chondroadherin His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human Chondroadherin was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 33-38 kDa.

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Background: Chondroadherin

Chondroadherin (CHAD), also known as SLRR4A, is a 38-kDa secreted protein belonging to the small leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function of the ECM (1). CHAD is highly expressed in cartilaginous tissues, with lower expression levels found in bone, tendon, and eye (2-4). Mature human CHAD consists of a 337 amino acid (aa) protein that contains eleven leucine-rich repeats (LRRs) including nine tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich regions (5). CHAD interacts with collagen II and mediates signaling between chondrocytes and the ECM by binding to the alpha 2 beta 1 integrin, heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In addition, CHAD interacts with both N- and C-terminal globular domains of type VI collagen (10). Human CHAD shares 95% and 94% aa sequence identity with mouse and rat CHAD, respectively.

References
  1. Neame, P.J. et al. (1994) J. Biol. Chem. 269:21547.
  2. Larson, T. et al. (1991) J. Biol. Chem. 266:20428.
  3. Mizuno, M. et al. (1996) Calcif. Tissue Int. 59:163.
  4. Johnson, J.M. et al. (2006) Mol. Vis. 12:1057.
  5. Månsson, B. et al. (2001) J. Biol. Chem. 276:32883.
  6. Paracuellos, P. et al. (2017) Matrix Biol. 63:106.
  7. Batista, M. et al. (2014) Matrix Biol. 38:84.
  8. Camper, L. et al. (1997) J. Cell Biol. 138:1159.
  9. Haglund, L. et al. (2013) J. Biol. Chem. 288:995.
  10. Wiberg, C. et al. (2002) J. Biol. Chem. 277:49120.
Entrez Gene IDs
1101 (Human); 12643 (Mouse); 29195 (Rat)
Alternate Names
CHAD; chondroadherin proteoglycan; Chondroadherin; SLRR4A; SLRR4ACartilage leucine-rich protein

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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