Recombinant Human CL-K1/COLEC11 Protein, CF

Catalog # Availability Size / Price Qty
5025-CL-050
Product Details
Citations (1)
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Recombinant Human CL-K1/COLEC11 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.
Source
Mouse myeloma cell line, NS0-derived human CL-K1/COLEC11 protein
Gln26-Met271, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln26 predicted
Predicted Molecular Mass
27 kDa
SDS-PAGE
36 kDa, reducing conditions

Product Datasheets

5025-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CL-K1/COLEC11

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain an N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the blood (4, 5). Alternative splicing may generate multiple isoforms with deletions or substitutions in the N-terminal and collagen-like domains (6). CL-K1 is widely expressed, notably in renal proximal tubules, bronchial glands, and gastrointestinal mucosa (5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature human CL-K1 shares 93% amino acid sequence identity with mouse and rat CL-K1. Within the CRD, human CL-K1 shares 55% aa sequence identity with CL-L1 and 26% - 33% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

References
  1. Gupta, G. and A. Surolia, 2007, Bioessays 29:452.
  2. van de Wetering, J.K. et al., 2004, Eur. J. Biochem. 271:1229.
  3. Holmskov, U. et al., 2003, Annu. Rev. Immunol. 21:547.
  4. Keshi, H. et al., 2006, Microbiol. Immunol. 50:1001.
  5. Motomura, W. et al., 2008, J. Histochem. Cytochem. 56:243.
  6. SwissProt # Q9BWP8.

Long Name
Collectin Kidney 1
Entrez Gene IDs
78989 (Human); 71693 (Mouse)
Alternate Names
CLK1; CL-K1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb; COLEC11; collectin kidney I; Collectin kidney protein 1; collectin sub-family member 11; collectin-11; DKFZp686N1868; MGC3279

Citation for Recombinant Human CL-K1/COLEC11 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome.
    Authors: Rooryck C, Diaz-Font A, Osborn DP
    Nat. Genet., 2011;43(3):197-203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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