Recombinant Human CL-K1/COLEC11 Protein, CF

Catalog # Availability Size / Price Qty
5025-CL-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human CL-K1/COLEC11 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.
Source
Mouse myeloma cell line, NS0-derived human CL-K1/COLEC11 protein
Gln26-Met271, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln26 predicted
Predicted Molecular Mass
27 kDa
SDS-PAGE
36 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5025-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CL-K1/COLEC11

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain an N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the blood (4, 5). Alternative splicing may generate multiple isoforms with deletions or substitutions in the N-terminal and collagen-like domains (6). CL-K1 is widely expressed, notably in renal proximal tubules, bronchial glands, and gastrointestinal mucosa (5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature human CL-K1 shares 93% amino acid sequence identity with mouse and rat CL-K1. Within the CRD, human CL-K1 shares 55% aa sequence identity with CL-L1 and 26% - 33% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

References
  1. Gupta, G. and A. Surolia, 2007, Bioessays 29:452.
  2. van de Wetering, J.K. et al., 2004, Eur. J. Biochem. 271:1229.
  3. Holmskov, U. et al., 2003, Annu. Rev. Immunol. 21:547.
  4. Keshi, H. et al., 2006, Microbiol. Immunol. 50:1001.
  5. Motomura, W. et al., 2008, J. Histochem. Cytochem. 56:243.
  6. SwissProt # Q9BWP8.

Long Name
Collectin Kidney 1
Entrez Gene IDs
78989 (Human); 71693 (Mouse)
Alternate Names
CLK1; CL-K1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb; COLEC11; collectin kidney I; Collectin kidney protein 1; collectin sub-family member 11; collectin-11; DKFZp686N1868; MGC3279

Citation for Recombinant Human CL-K1/COLEC11 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome.
    Authors: Rooryck C, Diaz-Font A, Osborn DP
    Nat. Genet., 2011;43(3):197-203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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