Recombinant Human CLEC-12B Fc Chimera Protein, CF Summary
Accession # Q2HXU8-1
When Recombinant Human CLEC12B Fc Chimera (Catalog # 1652-CL) is coated onto a microplate at 4 µg/mL, Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) binds with an ED50 of 1-6 μg/mL.
2 μg/lane of Recombinant Human CLEC12B was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 54-66 kDa and 110-135 kDa, respectively.
C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).
- Hoffmann, S. et al. (2007) J. Biol. Chem. 282:22370.
- Huysamen, C. et al. (2009) FEMS Microbiol. Lett. 290:121.
- Monteiro, J.T. and B. Lepenies (2017) Viruses 9:59.
- van den Berg, L.M. et al. (2012) Ann N Y Acad Sci. 1253:149.
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