Recombinant Human CLEC-12B Fc Chimera Protein, CF

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Recombinant Human CLEC-12B Fc Chimera Protein, CF Binding Activity
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Product Details

Recombinant Human CLEC-12B Fc Chimera Protein, CF Summary

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human CLEC12B Fc Chimera is immobilized at 4 µg/mL (100 µL/well), Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) binds with an ED50 of 1-6 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human CLEC12B protein
MDHuman IgG1
Accession # Q2HXU8-1
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
54-66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human CLEC12B Fc Chimera (Catalog # 1652-CL) is coated onto a microplate at 4 µg/mL, Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) binds with an ED50 of 1-6 μg/mL.


2 μg/lane of Recombinant Human CLEC12B was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 54-66 kDa and 110-135 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: CLEC12B

C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).

  1. Hoffmann, S. et al. (2007) J. Biol. Chem. 282:22370.
  2. Huysamen, C. et al. (2009) FEMS Microbiol. Lett. 290:121.
  3. Monteiro, J.T. and B. Lepenies (2017) Viruses 9:59.
  4. van den Berg, L.M. et al. (2012) Ann N Y Acad Sci. 1253:149.
Long Name
C-type Lectin Domain Family 12, Member B
Entrez Gene IDs
387837 (Human); 71183 (Mouse); 502900 (Rat)
Alternate Names
CLEC12B; C-type lectin domain family 12 member B; C-type lectin domain family 12, member B; Macrophage Antigen H; UNQ5782; UNQ5782/PRO16089


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