Recombinant Human CLEC17A Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10147-CL-50
10147-CL-050
Recombinant Human CLEC17A Fc Chimera Protein, CF Bioactivity
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Recombinant Human CLEC17A Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 for this effect is 1.5-9 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CLEC17A protein
MDHuman IgG1
(Pro100-Lys330)
IEGR Mouse CLEC17A
(Lys194-Cys378)
Accession # Q6ZS10-1
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48 kDa
SDS-PAGE
54-66 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10147-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Immobilized Recombinant Human CLEC17A Fc Chimera (Catalog #10147-CL) supports the adhesion of the MCF‑7 human breast cancer cells. TheED50 for this effect is 1.5-9 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human CLEC17A Fc Chimera (Catalog # 10147-CL) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® Blue staining, showing bands at 54-66 kDa and 110-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC17A

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2).  It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca2+-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

References
  1. Graham, S. A. et al. (2009) J. Biol. Chem. 284:18537.
  2. Breiman, A. et al. (2016) Oncotarget 7:14064.
  3. Koh, G. et al. (2011) BMC Bioinformatics 12:S5.
  4. Zelensky AN. et al. (2005) FEBS Journal 272:6179.
Long Name
C-type Lectin Domain Family 17, Member A
Entrez Gene IDs
388512 (Human)
Alternate Names
CLEC17A; C-type lectin domain family 17, member A; Prolectin

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