Recombinant Human CLEC2L Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10204-CL-050
Recombinant Human CLEC2L Fc Chimera Protein, CF Binding Activity
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Recombinant Human CLEC2L Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin-3 (Catalog # 1154-GA) that produces 50% of the optimal binding response is 1.5-12 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CLEC2L protein
MD Human IgG2A
(Pro100-Lys333)
IEGR Human CLEC2L
(Ser90-Thr214)
Accession # P0C7M8-1
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa
SDS-PAGE
39-44 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10204-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Galectin-3 (Catalog # 1154-GA) binds with an ED50 of 1.5-12 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-44 kDa and 80-90 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC2L

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

References
  1. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
  2. Vogler, I. and A. Steinle (2011) J. Innate Immune. 3:227.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.
  5. Rosen, D.B. et al. (2008) J. Immunol. 180:6508.
  6. Lysenko, O. et al. (2013) PloS One. 8:e65345.
Long Name
C-type Lectin Domain Family 2, Member L
Entrez Gene IDs
154790 (Human); 665180 (Mouse); 296985 (Rat)
Alternate Names
CLEC2L; C-type lectin domain family 2 member L; C-type lectin domain family 2, member L

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