Recombinant Human CLEC2L Fc Chimera Protein, CF Summary
Accession # P0C7M8-1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Galectin-3 (Catalog # 1154-GA) binds with an ED50 of 1.5-12 μg/mL.
2 μg/lane of Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-44 kDa and 80-90 kDa, respectively.
C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.
- Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
- Vogler, I. and A. Steinle (2011) J. Innate Immune. 3:227.
- Welte, S. et al. (2006) Nat. Immunol. 7:1334.
- Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.
- Rosen, D.B. et al. (2008) J. Immunol. 180:6508.
- Lysenko, O. et al. (2013) PloS One. 8:e65345.
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