Recombinant Human COCO Protein

Carrier Free

Catalog # Availability Size / Price Qty
3047-CC-050/CF

With Carrier

Catalog # Availability Size / Price Qty
3047-CC-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (4)
FAQs
Reviews (1)

Recombinant Human COCO Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.15-0.75 µg/mL in the presence of 50 ng/mL of rhBMP-4.
Source
E. coli-derived human COCO protein
Arg23-Ala189, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
18.2 kDa (monomer)

Product Datasheets

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3047-CC (with carrier)

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3047-CC/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3047-CC

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3047-CC/CF

Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: COCO

COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN Domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1 - 3). BMPs play important roles in tissue morphogenesis and development processes (4, 5, 6). The human COCO cDNA encodes a 189 amino acid (aa) precursor with a 22 aa signal sequence (2, 7). COCO has eight Cys residues in the cysteine-knot which places it in the CAN subfamily of BMP antagonists along with the other DAN family proteins (1). Human COCO shares 60% and 24% aa sequence identity with mouse and Xenopus COCO, respectively. It shares 17%, 20%, 25%, and 22% aa sequence identity with human DAN, Gremlin, PRDC, and Cerberus, respectively. In Xenopus embryonal development, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO binds and inhibits Xnr1, BMP-4, Activin, and Wnt-8 (7). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8). COCO may cooperate with Nodal in gastrulation and embryonic left-right axis formation (5, 8).

References
  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
  3. Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
  4. Baron, M.H. (2005) Exp. Hematol. 33:1015.
  5. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  6. De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
  7. Bell, E. et al. (2003) Development 130:1381.
  8. Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
Entrez Gene IDs
199699 (Human); 23863 (Mouse); 685719 (Rat)
Alternate Names
CER2CRL2; Cerberus 2; Cerberus like-2; cerberus-like 2; Cerberus-like protein 2; CERL2; cerl-2; CKTSF1B3; CKTSF1B3MGC126849; COCO; Cysteine knot superfamily 1, BMP antagonist 3; DAN domain family member 5; DAN domain family, member 5; DAND5; Dante; DTE; FLJ38607; GREM3; GREM3cerberus 2; gremlin-3; SP1

Citations for Recombinant Human COCO Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. ABCA4 Variant c.5714+5G>A in Trans With Null Alleles Results in Primary RPE Damage
    Authors: Sajovic, J;Meglic, A;Corradi, Z;Khan, M;Maver, A;Vidmar, MJ;Hawlina, M;Cremers, FPM;Fakin, A;
    Investigative ophthalmology & visual science
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Bioassay
  2. Correction of the Splicing Defect Caused by a Recurrent Variant in ABCA4 (c.769-784C&gt;T) That Underlies Stargardt Disease
    Authors: TZ Tomkiewicz, SE Nieuwenhui, FPM Cremers, A Garanto, RWJ Collin
    Cells, 2022-12-07;11(24):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. COCO/DAND5 inhibits developmental and pathological ocular angiogenesis
    Authors: N Popovic, E Hooker, A Barabino, A Flamier, F Provost, M Buscarlet, G Bernier, B Larrivée
    Embo Molecular Medicine, 2021-02-15;0(0):e12005.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Identification and Rescue of Splice Defects Caused by Two Neighboring Deep-Intronic ABCA4 Mutations Underlying Stargardt Disease
    Authors: S Albert, A Garanto, R Sangermano, M Khan, NM Bax, CB Hoyng, J Zernant, W Lee, R Allikmets, RWJ Collin, FPM Cremers
    Am. J. Hum. Genet., 2018-03-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human COCO Protein
By Anonymous on 01/01/2022
Application: Stem/Immune cell maintenance or differentiation

COCO promote the formation of organoid