Recombinant Human Collagen XV alpha 1/COL15A1 Protein, CF

Catalog # Availability Size / Price Qty
9854-CL-050

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Recombinant Human Collagen XV alpha 1/COL15A1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Collagen XV  alpha 1/COL15A1, immobilized at 0.5 μg/mL, is able to significantly enhance neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human Collagen XV alpha 1/COL15A1 protein
Hemagglutinin Tag
(YPYDVPDYA)
Human COL15A1
(Asn1133-Ser1381)
Accession # P39059


Accession #
N-terminal Sequence
Analysis
Tyr
Structure / Form
Noncovalently-linked homotrimer
Predicted Molecular Mass
29 kDa
SDS-PAGE
26-36 kDa, reducing conditions

Product Datasheets

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9854-CL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9854-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Collagen XV alpha 1/COL15A1

Recombinant human Collagen XV alpha 1/COL15A1 is the C-terminal endostatin-like domain (amino acids 1133-1381) of human Collagen XV. Collagen XV is one of the two members of a vertebrate collagen family termed multiplexins. The other member of this family is Collagen XVIII. This family is characterized by a collagen triple helix region that is interrupted by several non-collagenous stretches, as well as a C-terminal non-triple helical NC1 domain (1, 2). The NC1 domain is composed of three functionally distinct regions: an association region in its most N-terminal part that trimerizes NC1 monomers, a protease-sensitive hinge region, and the C-terminal globular endostatin (ES) domain, or in the case of Collagen XV, an ES-like domain (2, 3). Both Collagen XV and Collagen XVIII are located in the basement membrane or the basement zone. They show anti-angiogenic and anti-tumoral properties (4), and play an important role in tissue homeostasis in liver, kidney, eye and the central nervous system (5). The Drosophila multiplexin (Dmp) is shown to modulate axon pathfinding accuracy in motor pathways (1). Within amino acids 1133-1381, the human protein shares 74% sequence homology with its mouse counterpart, and 73% sequence homology with that of the rat. This ES-like domain in Collagen XV show approximately 60% sequence identity with the endostatin domain of type XVIII Collagen. Studies have shown that endostatins derived from Collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity (6).

References
  1. Meyer, F. and Moussian B. (2009) Develop. Growth Differ. 51:483.
  2. Marneros, A. G. and Olsen B. R. (2001) Matrix Biol. 20:337.
  3. Wirz, J. et al. (2011) Matrix Biol. 30:9.
  4. Ricard-Blum, S. and Ballut L. (2011) Front Biosci 16:674.
  5. Heljasvaara, R. et al. (2016) Matrix Biol. 57-58:55.
  6. Sasaki, T. et al. (2000) J. Mol. Biol. 301:1179.
Long Name
Collagen Type XV Alpha 1 Chain
Entrez Gene IDs
1306 (Human); 12819 (Mouse); 298069 (Rat)
Alternate Names
alpha-1 polypeptide; COL15A1; Collagen 15 alpha 1; collagen alpha-1(XV) chain; Collagen Type XV Proteoglycan; Collagen XV alpha 1; Collagen XV, Alpha-1 Polypeptide; collagen, type XV, alpha 1; Endostatin-XV; FLJ38566; Restin

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