Recombinant Human COMP His-tag Protein, CF Summary
Gln21-Ala757 (Ala256Arg), with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Human COMP/Thrombospondin‑5 (Catalog # 3134-CPB) at 10 µg/mL (100 µL/well) induces more than 40% cell adhesion.
2 μg/lane of Recombinant Human COMP/Thrombospondin‑5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-118 kDa and 500 - 550 kDa, respectively.
Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous coiled‑coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type‑3 repeat, results in diminished calcium binding ability (13, 14).
- Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
- Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005.
- Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
- Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
- Newton, G. et al. (1994) Genomics, 24:435.
- DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
- Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
- Ozbek, S. et al. (2002) EMBO J. 21:5960.
- Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655.
- Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
- Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
- Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
- Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547.
- Hou, J. et al. (2000) Cell Calcium 27:309.
Citations for Recombinant Human COMP His-tag Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP
Authors: L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu
J. Exp. Clin. Cancer Res., 2019;38(1):170.
Sample Types: Whole Cells
HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways
Authors: Q Li, C Wang, Y Wang, L Sun, Z Liu, L Wang, T Song, Y Yao, Q Liu, K Tu
J. Exp. Clin. Cancer Res., 2018;37(1):231.
Sample Types: Whole Cells
Molecular synergy in biolubrication: The role of cartilage oligomeric matrix protein (COMP) in surface-structuring of lubricin
Authors: A Raj, M Wang, C Liu, L Ali, NG Karlsson, PM Claesson, A D?dinait?
J Colloid Interface Sci, 2017;495(0):200-206.
Deficiency of Thrombospondin-4 in Mice Does Not Affect Skeletal Growth or Bone Mass Acquisition, but Causes a Transient Reduction of Articular Cartilage Thickness.
Authors: Jeschke A, Bonitz M, Simon M, Peters S, Baum W, Schett G, Ruether W, Niemeier A, Schinke T, Amling M
PLoS ONE, 2015;10(12):e0144272.
Sample Types: Whole Cells
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