Recombinant Human Complement Factor H-related 1 His-tag, CF

Catalog # Availability Size / Price Qty
4247-CH-050
Recombinant Human Complement Factor H-related 1 His-tag, CF Binding Activity
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Recombinant Human Complement Factor H-related 1 His-tag, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Complement Factor H-related 1/CFHR1 is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Mouse Complement Component C3d that produces 50% of the optimal binding response is 40-240 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Complement Factor H-related 1/CFHR1 protein
Glu19-Ala328, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Glu19
Predicted Molecular Mass
37 kDa
SDS-PAGE
39-48 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4247-CH

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human Complement Factor H-related 1/CFHR1 His-tag (Catalog # 4247-CH) is immobilized at 1 μg/mL, 100 μL/well, Biotinylated Recombinant Mouse Complement Component C3d binds with an ED50 of 40‑240 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Complement Factor H-related 1/CFHR1 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands under reducing conditions at 41 kDa and 45 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H-related 1/CFHR1

Complement factor H-related protein 1 (CFHR1) is a ~40 kDa secreted, primarily homodimeric member of the factor H family of glycoproteins (1). CFHR1 is produced by hepatocytes and circulates as two differentially glycosylated isoforms. The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (2). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. CFHR1 contains 5 SCRs. Although they are considered group 1 CFHRs based on conserved N-termini (2), compared to CFHR1, human CFHR2 and CFHR5 show 67.5% and 34.5% aa identity, respectively. All CFHRs, including CHFR1, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b (2). CFHR1 inhibits complement C5 convertase activity (4). A common CFHR1/CFHR3 genetic deletion has been implicated as being protective against age-related macular degeneration (5) and nephropathy (6).  The same deletion is associated with development of factor H auto-antibodies and susceptibility to atypical hemolytic uremic syndrome due to the deletion of CFHR1 (7-8). Additionally, CFHR1 is recruited to the surface of pathogens which is suggested to result in evasion of host complement attack (9).

References
  1. van Beek, A.E. et al. (2017) Front. Immunol. 18:1328.
  2. Skerka, C. et al. (2013) Mol. Immunol. 56:170.
  3. Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
  4. Heinen, S. et al. (2009) Blood 114:2439.
  5. Hughes, A.E. et al. (2006) Nat. Genet. 38:1173.
  6. Gharavi, A.G. et al. (2011) Nat. Genet. 43:321.
  7. Abarrategui-Garrido, C. (2009) Blood 114:4261.
  8. Munch, J. et al. (2017) Clin. Kidney J. 10:742.
  9. Reuter, M. et al. (2010) J. Biol. Chem. 285:38473.
Entrez Gene IDs
3078 (Human)
Alternate Names
BTA; CFHL1FHR-1; CFHLCFHR1P; CFHR1; complement factor H-related 1 pseudogene; Complement Factor Hrelated 1; Complement Factor H-related 1; complement factor H-related protein 1; FHR1; FHR1CFHL1P; H factor (complement)-like 1; H factor (complement)-like 2; H factor-like protein 1; H36-1; H36-2; H-factor-like 1; HFL1H36; HFL2; MGC104329

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