Recombinant Human Complement Factor H-related 2 His-tag, CF Summary
Glu19-Glu268, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL, 100 µL/well, Recombinant HumanComplement Factor H-related 2 (Catalog # 5484-CH) binds with an ED50 of25-150 ng/mL.
2 μg/lane of Recombinant Human Complement Factor H-related 2/CFHR2 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at ~31 kDa and ~36 kDa under reducing conditions.
Background: Complement Factor H-related 2/CFHR2
Complement factor H-related protein 2 (CFHR2) is a ~30 kDa secreted, primarily homodimeric member of the factor H family of glycoproteins (1). CFHR2 is produced by hepatocytes and circulates as two differentially glycosylated isoforms (2). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (CFHR-1 to -5) (3). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (4). CFHRs are exclusively composed of individually folded protein domains termed short consensus repeats (SCRs) or complement control modules. CFHR2 contains 4 SCRs. Although considered group 1 CFHRs based on conserved N-termini (1), compared to CFHR2, human CFHR1 and CFHR5 show 67.5% and 34.5% aa identity, respectively. All CFHRs are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation (3). CFHR2 inhibits the C3 convertase cleavage amplification loop by inhibiting terminal complement complex assembly (5). CFHR2 polymorphisms and deficiency were identified to correlate with risk of age-related macular degeneration (AMD) (6-8) implicating CFHR2 in human disease.
- van Beek, A.E. et al. (2017) Front. Immunol. 18:1328.
- Skerka, C. et al. (1992) J. Immunol. 148:3313.
- Skerka, C. et al. (2013) Mol. Immunol. 56:170.
- Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
- Eberhardt, H.U. et al. (2013) PLOS One 8:e78617.
- Zhang, H. et al. (2008) BMC Med. Genet. 9:51.
- Kubista, K.E. et al. (2011) Mol. Vis. 17:2080.
- Cantsilieris, S. et al. (2018) Proc. Natl. Acad. Sci. 115:E4433.
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