Recombinant Human Complement Factor H-related 2 His-tag, CF

Catalog # Availability Size / Price Qty
5484-CH-050
Complement Factor H-related 2/CFHR2 Protein Bioactivity
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Recombinant Human Complement Factor H-related 2 His-tag, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H-related 2/CFHR2 binds with an ED50 of 25-150 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Complement Factor H-related 2/CFHR2 protein
Glu19-Glu268, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Glu19
Predicted Molecular Mass
30 kDa
SDS-PAGE
30-38 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5484-CH

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL, 100 µL/well, Recombinant HumanComplement Factor H-related 2 (Catalog # 5484-CH) binds with an ED50 of25-150 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Complement Factor H-related 2/CFHR2 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at ~31 kDa and ~36 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H-related 2/CFHR2

Complement factor H-related protein 2 (CFHR2) is a ~30 kDa secreted, primarily homodimeric member of the factor H family of glycoproteins (1). CFHR2 is produced by hepatocytes and circulates as two differentially glycosylated isoforms (2). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (CFHR-1 to -5) (3). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (4). CFHRs are exclusively composed of individually folded protein domains termed short consensus repeats (SCRs) or complement control modules. CFHR2 contains 4 SCRs. Although considered group 1 CFHRs based on conserved N-termini (1), compared to CFHR2, human CFHR1 and CFHR5 show 67.5% and 34.5% aa identity, respectively. All CFHRs are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation (3). CFHR2 inhibits the C3 convertase cleavage amplification loop by inhibiting terminal complement complex assembly (5). CFHR2 polymorphisms and deficiency were identified to correlate with risk of age-related macular degeneration (AMD) (6-8) implicating CFHR2 in human disease.

References
  1. van Beek, A.E. et al. (2017) Front. Immunol. 18:1328.
  2. Skerka, C. et al. (1992) J. Immunol. 148:3313.
  3. Skerka, C. et al. (2013) Mol. Immunol. 56:170.
  4. Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
  5. Eberhardt, H.U. et al. (2013) PLOS One 8:e78617.
  6. Zhang, H. et al. (2008) BMC Med. Genet. 9:51.
  7. Kubista, K.E. et al. (2011) Mol. Vis. 17:2080.
  8. Cantsilieris, S. et al. (2018) Proc. Natl. Acad. Sci. 115:E4433.
Entrez Gene IDs
3080 (Human)
Alternate Names
CFHL2; CFHL2FHR-2; CFHR2; Complement Factor Hrelated 2; Complement Factor H-related 2; factor H-related gene 2; FHR2; FHR2DDESK59; H factor (complement)-like 3; H factor-like 3; H factor-like protein 2; HFL3; HFL3complement factor H-related protein 2

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