Recombinant Human Complement Factor H-related 4/CFHR4, CF Summary
Gln19-Glu331, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human Complement Factor H-related 4/CFHR4 (Catalog # 5980-CH) binds with an ED50of 1-6 ng/mL.
2 μg/lane of Recombinant Human Complement Factor H‑related 4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~42 kDa and ~40 kDa, respectively.
Background: Complement Factor H-related 4/CFHR4
Complement factor H-related protein 4 (CFHR4) is a secreted glycoprotein member of the factor H family of glycoproteins (1). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (1). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (2). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. All CFHRs, including CFHR4, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b and C3d (1, 3). CFHR4 activates complement through its interaction with C3b (4). CFHR4 can also bind to native (pentameric) C-reactive protein (pCRP) through its first SCR domain and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance (5). CFHR4 is synthesized as 2 isoforms by hepatocytes and circulates in plasma. The longer 86 kDa alternative splice form termed FHR4A includes a duplication insertion of 247 aa. The CFHR4 product represents the shorter isoform, FHR4B, containing 5 of 9 SCRs (6).
- Skerka, C. et al. (2013) Mol. Immunol. 56:170.
- Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
- Hellwage, J. et al. (1999) FEBS Lett. 462:345.
- Hebecker, M. and J. Jozsi (2011) J. Biol. Chem. 287:19528.
- Mihlan, M. et al. (2009) Mol. Immunol. 46:335.
- Jozsi, M. et al. (2005) Eur. J. Hum. Genet. 13:321.
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