Recombinant Human Complement Factor H-related 4/CFHR4, CF

His-tag
Catalog # Availability Size / Price Qty
5980-CH-050
Recombinant Human Complement Factor H-related 4/CFHR4, CF Binding Activity
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Recombinant Human Complement Factor H-related 4/CFHR4, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H‑related 4/CFHR4 binds with an ED50 of 1-6 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Complement Factor H-related 4/CFHR4 protein
Gln19-Glu331, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln19 inferred from enzymatic pyroglutamate treatment revealing Glu20.
Predicted Molecular Mass
37 kDa
SDS-PAGE
39-45 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5980-CH

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human Complement Factor H-related 4/CFHR4 (Catalog # 5980-CH) binds with an ED50of 1-6 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Complement Factor H‑related 4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~42 kDa and ~40 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H-related 4/CFHR4

Complement factor H-related protein 4 (CFHR4) is a secreted glycoprotein member of the factor H family of glycoproteins (1). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (1). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (2). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. All CFHRs, including CFHR4, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b and C3d (1, 3). CFHR4 activates complement through its interaction with C3b (4). CFHR4 can also bind to native (pentameric) C-reactive protein (pCRP) through its first SCR domain and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance (5). CFHR4 is synthesized as 2 isoforms by hepatocytes and circulates in plasma. The longer 86 kDa alternative splice form termed FHR4A includes a duplication insertion of 247 aa. The CFHR4 product represents the shorter isoform, FHR4B, containing 5 of 9 SCRs (6).

References
  1. Skerka, C. et al. (2013) Mol. Immunol. 56:170.
  2. Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
  3. Hellwage, J. et al. (1999) FEBS Lett. 462:345.
  4. Hebecker, M. and J. Jozsi (2011) J. Biol. Chem. 287:19528.
  5. Mihlan, M. et al. (2009) Mol. Immunol. 46:335.
  6. Jozsi, M. et al. (2005) Eur. J. Hum. Genet. 13:321.
Entrez Gene IDs
10877 (Human)
Alternate Names
complement factor H-related protein 4; CFHL4; CFHR4; Complement Factor Hrelated 4; Complement Factor H-related 4; FHR4; FHR-4

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