Recombinant Human Complement Factor H-related 5/CFHR5, CF

Catalog # Availability Size / Price Qty
3845-F5-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (5)
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Recombinant Human Complement Factor H-related 5/CFHR5, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the binding of biotinylated Recombinant Human Complement Factor H-related 5/CFHR5 to immobilized Recombinant Human C‑Reactive Protein/CRP (Catalog # 1707-CR). McRae, J.L. et al. (2005) J. Immunol. 174:6250.
Source
Mouse myeloma cell line, NS0-derived human Complement Factor H-related 5/CFHR5 protein
Glu19-Glu569, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Glu19
Predicted Molecular Mass
64 kDa
SDS-PAGE
73 kDa, reducing conditions

Product Datasheets

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3845-F5

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3845-F5

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Complement Factor H-related 5/CFHR5

The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). FHR-5 has been identified initially as a universal component of complement deposits (1), and detected in glomerular immune deposits (4). The pattern of deposits is similar to other complement components, suggesting that FHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. Its biological function is not understood fully. FHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association (5). Weak factor I-dependent cofactor activity for C3b cleavage has also been observed (5).

References
  1. McRae, J. L. et al. (2001) J. Biol. Chem. 276:6747.
  2. Zipfel, P. F. et al. (2002) Biochem. Soc. Trans. 30:971.
  3. McRae, J. L. et al. (2002) Genetica. 114:157.
  4. Murphy, B. et al. (2002) Am. J. Kidney. Dis. 39:24.
  5. McRae, J. L. et al. (2005) J. Immunol. 174:6250.
Entrez Gene IDs
81494 (Human)
Alternate Names
CFHL5; CFHL5factor H-related protein 5; CFHR5; Complement Factor Hrelated 5; Complement Factor H-related 5; FHR5; FHR-5complement factor H-related protein 5; FHR5MGC133240; FLJ10549

Citations for Recombinant Human Complement Factor H-related 5/CFHR5, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Elevated plasma complement factor H related 5 protein is associated with venous thromboembolism
    Authors: Iglesias, MJ;Sanchez-Rivera, L;Ibrahim-Kosta, M;Naudin, C;Munsch, G;Goumidi, L;Farm, M;Smith, PM;Thibord, F;Kral-Pointner, JB;Hong, MG;Suchon, P;Germain, M;Schottmaier, W;Dusart, P;Boland, A;Kotol, D;Edfors, F;Koprulu, M;Pietzner, M;Langenberg, C;Damrauer, SM;Johnson, AD;Klarin, DM;Smith, NL;Smadja, DM;Holmström, M;Magnusson, M;Silveira, A;Uhlén, M;Renné, T;Martinez-Perez, A;Emmerich, J;Deleuze, JF;Antovic, J;Soria Fernandez, JM;Assinger, A;Schwenk, JM;Souto Andres, JC;Morange, PE;Butler, LM;Trégouët, DA;Odeberg, J;
    Nature communications
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot, ELISA
  2. Glomerular Complement Factor H-Related Protein 5 is Associated with Histologic Injury in Immunoglobulin A Nephropathy
    Authors: WY Guo, LJ Sun, HR Dong, GQ Wang, XY Xu, ZR Zhao, H Cheng
    Kidney international reports, 2020-11-21;6(2):404-413.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Control
  3. Glomerular Complement Factor H-Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment
    Authors: NR Medjeral-T, H Moffitt, HJ Lomax-Brow, N Constantin, T Cairns, HT Cook, MC Pickering
    Kidney Int Rep, 2019-06-19;4(10):1387-1400.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. FHR-1 Binds to C-Reactive Protein and Enhances Rather than Inhibits Complement Activation
    Authors: ÁI Csincsi, Z Szabó, Z Bánlaki, B Uzonyi, M Cserhalmi, É Kárpáti, A Tortajada, JJE Caesar, Z Prohászka, TS Jokiranta, SM Lea, S Rodríguez, M Józsi
    J. Immunol., 2017-05-22;0(0):.
    Applications: Bioassay
  5. Factor H-related protein 5 interacts with pentraxin 3 and the extracellular matrix and modulates complement activation.
    Authors: Csincsi A, Kopp A, Zoldi M, Banlaki Z, Uzonyi B, Hebecker M, Caesar J, Pickering M, Daigo K, Hamakubo T, Lea S, Goicoechea de Jorge E, Jozsi M
    J Immunol, 2015-04-08;194(10):4963-73.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay

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