Recombinant Human Complement Factor H-related 5/CFHR5, CF

R&D Systems | Catalog # 3845-F5

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Complement Factor H-related 5/CFHR5 (3845-F5)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_110414

Applications

Binding Activity
View all Complement Factor H-related 5/CFHR5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Complement Factor H-related 5/CFHR5 protein
Glu19-Glu569, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu19

Predicted Molecular Mass

64 kDa

SDS-PAGE

73 kDa, reducing conditions

Activity

Measured by its ability to inhibit the binding of biotinylated Recombinant Human Complement Factor H-related 5/CFHR5 to immobilized Recombinant Human C‑Reactive Protein/CRP (Catalog # 1707-CR). McRae, J.L. et al. (2005) J. Immunol. 174:6250.

Formulation, Preparation, and Storage

3845-F5
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H-related 5/CFHR5

The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). FHR-5 has been identified initially as a universal component of complement deposits (1), and detected in glomerular immune deposits (4). The pattern of deposits is similar to other complement components, suggesting that FHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. Its biological function is not understood fully. FHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association (5). Weak factor I-dependent cofactor activity for C3b cleavage has also been observed (5).

References

  1. McRae, J. L. et al. (2001) J. Biol. Chem. 276:6747.
  2. Zipfel, P. F. et al. (2002) Biochem. Soc. Trans. 30:971.
  3. McRae, J. L. et al. (2002) Genetica. 114:157.
  4. Murphy, B. et al. (2002) Am. J. Kidney. Dis. 39:24.
  5. McRae, J. L. et al. (2005) J. Immunol. 174:6250.

Alternate Names

CFHL5, CFHR5, Complement Factor Hrelated 5, FHR5

Entrez Gene IDs

81494 (Human)

Gene Symbol

CFHR5

UniProt

NP_110414

Additional Complement Factor H-related 5/CFHR5 Products

Product Documents for Recombinant Human Complement Factor H-related 5/CFHR5, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Complement Factor H-related 5/CFHR5, CF

For research use only

Citations for Recombinant Human Complement Factor H-related 5/CFHR5, CF

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