Recombinant Human Contactin-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
904-CN-100
Product Details
Citations (1)
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Recombinant Human Contactin-1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) NrCAM Fc Chimera (Catalog # 2034-NR) at 1 μg/mL (100 μL/well) can bind rhContactin-1 Fc Chimera with a linear range of 0.3-20 ng/mL. Measured by its ability to support the adhesion of C6 rat glioma cells. rhContactin Fc Chimera immobilized at 2 µg/mL, 100 µL/well will mediate >20% C6 cell adhesion.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Contactin-1 protein
Human Contactin-1
(Glu21-Ser993)
Accession # CAA79696
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Glu21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
135 kDa
SDS-PAGE
135-150 kDa, reducing conditions

Product Datasheets

904-CN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Contactin-1

Contactin-1 (CNTN1), also known as F3 and F11, is an approximately 135 kDa GPI-anchored member of the Contactin family of neuronal adhesion glycoproteins (1). Mature human Contactin-1 contains 6 Ig-like domains and 4 fibronectin type III-like domains, and it shares 96% amino acid (aa) sequence identity with mouse and rat Contactin-1 (2, 3). Alternative splicing generates an isoform with an 11 aa deletion N-terminal to the first Ig-like domain and another isoform that is substituted and truncated following the sixth Ig-like domain. A soluble form of Contactin-1 is released into the cerebrospinal fluid (4). Contactin-1 is expressed on neurons and their precursors at sites of glial cell contact with neuronal processes (5-7). Within paranodal regions of the axon, Contactin-1 associates in cis with the transmembrane protein Caspr (7). It binds in trans to other neuronal adhesion proteins (e.g. NrCAM, Neurofascin, Tenascin R), the phosphatase PTPRZ, and the chondroitin sulfate CS-E (8-13). It also binds and activates both Notch-1 and Notch-2 (14). Ligation of Contactin-1 promotes neurite outgrowth, adhesion to Schwann cells, axon myelination, and differentiation of oligodendrocytes and cerebellar granule neurons, but it can also negatively regulate neurogenesis in the ventricular zone
(4, 6-9, 12-14). In cancer, Contactin-1 is up-regulated by VEGF-C and is required for VEGF R3/Flt-4 induced tumor cell invasion and metastasis (15).

References
  1. Shimoda, Y. and K. Watanabe (2009) Cell Adh. Migr. 3:64.
  2. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
  3. Berglund, E.O. and B. Ranscht (1994) Genomics 21:571.
  4. Durbec, P. et al. (1992) J. Cell Biol. 117:877.
  5. Gennarini, G. et al. (1990) Acta Histochem. 38:65.
  6. Bizzoca, A. et al. (2012) Dev. Biol. 365:133.
  7. Boyle, M.E.T. et al. (2001) Neuron 30:385.
  8. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  9. Xenaki, D. et al. (2011) Development 138:519.
  10. Volkmer, H. et al. (1998) J. Cell Biol. 142:1083.
  11. Pesheva, P. et al. (1993) Neuron 10:69.
  12. Peles, E. et al. (1995) Cell 82:251.
  13. Mikami, T. et al. (2009) J. Biol. Chem. 284:4494.
  14. Hu, Q.-D. et al. (2003) Cell 115:163.
  15. Su, J.-L. et al. (2006) Cancer Cell 9:209.
Entrez Gene IDs
1272 (Human); 12805 (Mouse)
Alternate Names
CNTN1; contactin 1; Contactin1; Contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3

Citation for Recombinant Human Contactin-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Differentiation of human umbilical cord matrix mesenchymal stem cells into neural-like progenitor cells and maturation into an oligodendroglial-like lineage.
    Authors: Leite C, Silva N, Mendes S, Ribeiro A, de Faria J, Lourenco T, dos Santos F, Andrade P, Cardoso C, Vieira M, Paiva A, da Silva C, Cabral J, Relvas J, Graos M
    PLoS ONE, 2014;9(10):e111059.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Primary Antibodies

Human IgG1 Antibody

Human IgG1 Antibody

Recombinant Proteins

Reconstitution Buffers

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