Recombinant Human Contactin-2/TAG1 Protein, CF Summary
Leu29-Asn1012, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1 - 3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III-like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3 - 5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3 - 6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin-associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7 - 10). The axonal Contactin-2 interacts in cis with caspr2, and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).
- Wolfer, D. & R. J. Giger (1994) Swissprot accession # Q61330.
- Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
- Karagogeos, D. (2003) Front. Biosci.8:s1304.
- Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
- Denaxa, M. et al. (2005) Dev. Biol. 288:87.
- Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
- Traka, M. et al. (2002) J. Neurosci. 22:3016.
- Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
- Traka, M. et al. (2003) J. Cell Biol. 162:1161.
- Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Citations for Recombinant Human Contactin-2/TAG1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Neurofascin as target of autoantibodies in Guillain-Barre syndrome.
Authors: Pruss H, Schwab JM, Derst C, Gortzen A, Veh RW
Brain, 2011-01-27;134(0):e173; author.
Sample Types: Serum
Applications: ELISA (Capture)
Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals.
Authors: Derfuss T, Parikh K, Velhin S, Braun M, Mathey E, Krumbholz M, Kumpfel T, Moldenhauer A, Rader C, Sonderegger P, Pollmann W, Tiefenthaller C, Bauer J, Lassmann H, Wekerle H, Karagogeos D, Hohlfeld R, Linington C, Meinl E
Proc. Natl. Acad. Sci. U.S.A., 2009-04-28;106(20):8302-7.
Sample Types: Serum
Applications: ELISA (Capture)
Structural requirement of TAG-1 for retinal ganglion cell axons and myelin in the mouse optic nerve.
Authors: Chatzopoulou E, Miguez A, Savvaki M, Levasseur G, Muzerelle A, Muriel MP, Goureau O, Watanabe K, Goutebroze L, Gaspar P, Zalc B, Karagogeos D, Thomas JL
J. Neurosci., 2008-07-23;28(30):7624-36.
Sample Types: Whole Tissue
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