Recombinant Human Contactin-2/TAG1 Protein, CF

  (3 citations)     
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Product Details
Citations (3)
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  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the rhContactin-2 solution at
    10-25 µg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived human Contactin-2/TAG1 protein
    Leu29-Asn1012, with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu29
  • Predicted Molecular Mass
    108.9 kDa
  • SDS-PAGE
    145 kDa, reducing conditions
Product Datasheets

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1714-CN
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1 - 3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III-like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3 - 5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3 - 6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin-associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7 - 10). The axonal Contactin-2 interacts in cis with caspr2, and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

  • References:
    1. Wolfer, D. & R. J. Giger (1994) Swissprot accession # Q61330.
    2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
    3. Karagogeos, D. (2003) Front. Biosci.8:s1304.
    4. Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
    5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
    6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
    7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
    8. Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
    9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
    10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
  • Entrez Gene IDs:
    6900 (Human)
  • Alternate Names:
    Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Neurofascin as target of autoantibodies in Guillain-Barre syndrome.
    Authors: Pruss H, Schwab JM, Derst C, Gortzen A, Veh RW
    Brain, 2011;134(0):e173; author.
    Species: Human
    Sample Type: Serum
    Application: ELISA capture
  2. Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals.
    Authors: Derfuss T, Parikh K, Velhin S, Braun M, Mathey E, Krumbholz M, Kumpfel T, Moldenhauer A, Rader C, Sonderegger P, Pollmann W, Tiefenthaller C, Bauer J, Lassmann H, Wekerle H, Karagogeos D, Hohlfeld R, Linington C, Meinl E
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(20):8302-7.
    Species: Human
    Sample Type: Serum
    Application: ELISA capture
  3. Structural requirement of TAG-1 for retinal ganglion cell axons and myelin in the mouse optic nerve.
    Authors: Chatzopoulou E, Miguez A, Savvaki M, Levasseur G, Muzerelle A, Muriel MP, Goureau O, Watanabe K, Goutebroze L, Gaspar P, Zalc B, Karagogeos D, Thomas JL
    J. Neurosci., 2008;28(30):7624-36.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay

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Primary Antibodies
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His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 27  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB , Flow , AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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