Recombinant Human CRELD2 Protein, CF

R&D Systems | Catalog # 3555-CR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CRELD2 Protein (3555-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAH50675

Applications

Bioactivity
View all CRELD2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CRELD2 protein
Ala21-Leu353, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

36.7 kDa

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is < 10 μg/mL.

Formulation, Preparation, and Storage

3555-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRELD2

Cysteine-rich with EGF-like domain protein 2 (CRELD2) is an approximately 60 kDa glycoprotein that contains two EGF‑like domains and two FU domains (1). Mature human CRELD2 shares approximately 78% aa sequence identity with mouse and rat CRELD2. Alternative splicing of human CRELD2 generates additional isoforms with substitutions, deletions, and/or insertions in the C-terminal half of the molecule (2). The various isoforms are widely expressed in fetal and adult tissues (1‑3). CRELD2 localizes to the endoplasmic reticulum and Golgi and can also be secreted (3‑5). It is up‑regulated during the cellular stress‑induced unfolded protein response (UPR), cartilage and bone pathologies (MED and MCDS), and arsenic‑induced liver toxicity (4, 6‑8). CRELD2 interacts intracellularly with the alpha 4 and beta 2 subunits of the nicotinic acetylcholine receptor and inhibits cell surface expression of the receptor (3, 9). CRELD2 expression is up‑regulated in prostate cancer by dihydroxytestosterone and is down‑regulated following anti-androgen treatment (10).

References

  1. Rupp, P.A. et al. (2002) Gene 293:47.
  2. Maslen, C.L. et al. (2006) Gene 382:111.
  3. Ortiz, J.A. et al. (2005) J. Neurochem. 95:1585.
  4. Oh-hashi, K. et al. (2009) Biochem. Biophys. Res. Commun. 387:504.
  5. Oh-hashi, K. et al. (2011) FEBS Lett. 585:2481.
  6. Cameron, T.L. et al. (2011) PLoS ONE 6:e24600.
  7. Nundlall, S. et al. (2010) Cell Stress Chaperones 15:835.
  8. Nohara, K. et al. (2012) Toxicol. Sci. epub.
  9. Hosur, V. et al. (2009) J. Neurochem. 111:848.
  10. Jariwala, U. et al. (2007) Mol. Cancer 6:39.

Long Name

Cysteine-rich Protein With EGF-like Domains 2

Alternate Names

cysteine-rich with EGF-like domain protein 2, cysteine-rich with EGF-like domains 2, DKFZp667O055, MGC11256

Entrez Gene IDs

79174 (Human); 76737 (Mouse)

Gene Symbol

CRELD2

UniProt

AAH50675

Additional CRELD2 Products

Product Documents for Recombinant Human CRELD2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CRELD2 Protein, CF

For research use only

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