Recombinant Human CTRP4/C1qTNF4 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human CTRP4/C1qTNF4, immobilized at 1.25-2.5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
  • Source
    Mouse myeloma cell line, NS0-derived Leu17-Leu329, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu17
  • Predicted Molecular Mass
    34 kDa
  • SDS-PAGE
    34-39 kDa, reducing conditions
8357-TN
 
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
1 μg/lane ofRecombinant Human CTRP4/C1qTNF4 (Catalog # 8357-TN)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing R bands at 36.7 and 36.9 kDa, respectively.
Background: CTRP4/C1qTNF4
C1qTNF4, also known as CTRP4, is an approximately 34 kDa member of the C1q family of secreted proteins (1, 2). C1qTNF4 consists of a signal peptide followed by two tandem globular C1q domains (1). It is the only C1q family member with tandem C1q domains (1, 2). The second C1q domain contains an RGD peptide, suggesting that it could potentially interact with integrins (3). Mature human C1qTNF4 shares 95% and 94% amino acid sequence identity with mouse and rat C1qTNF4, respectively. Expression of human C1qTNF4 occurs most abundantly in brain and adipose tissue (1). Like other CTRP family members, C1qTNF4 can form dimers, trimers, hexamers, and high molecular weight oligomers following secretion (1, 4). Injection of recombinant C1qTNF4 into mice can reduce food intake, body weight, and ambulatory activity levels (1). C1qTNF4 has also been shown to promote human cancer cell survival in vitro (5).
  • References:
    1. Byerly, M.S. et al. (2014) J. Biol. Chem. 289:4055.
    2. Kishore, U. et al. (2004) Trends Immunol. 25:551.
    3. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
    4. Wong, G.W. et al. (2008) Biochem. J. 416:161.
    5. Li, Q. et al. (2011) Cancer Lett. 308:203.
  • Long Name:
    C1q And Tumor Necrosis Factor Related Protein 4
  • Entrez Gene IDs:
    114900 (Human); 67445 (Mouse); 311184 (Rat)
  • Alternate Names:
    C1q and tumor necrosis factor related protein 4; C1qTNF4; complement C1q tumor necrosis factor-related protein 4; complement-c1q tumor necrosis factor-related protein 4; CTRP4; CTRP4ZACRP4; ZACRP4
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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