Recombinant Human CTRP9/C1qTNF9 Protein, CF

Catalog # Availability Size / Price Qty
6537-TN-050
Recombinant Human CTRP9/C1qTNF9 Protein Binding Activity
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Recombinant Human CTRP9/C1qTNF9 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CTRP9/C1qTNF9 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CL-K1/COLEC11 that produces 50% of the optimal binding response is 0.035-0.21 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CTRP9/C1qTNF9 protein
Asn16-Pro333, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
34 kDa
SDS-PAGE
36-40 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6537-TN

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human CTRP9/C1qTNF9 Protein Binding Activity View Larger

When Recombinant Human CTRP9/C1qTNF9 (Catalog # 6537-TN) is coated at 0.5 μg/mL, 100 μL/well, Biotinylated Recombinant Human CL-K1/COLEC11 binds with an ED50 of 0.035-0.21 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Human C1qTNF9 shares 85% amino acid sequence identity with the mouse and rat orthologs. Both the mouse and human C1qTNF9 proteins are expressed in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). They have both also been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet‑induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).

References
  1. Wong, G.W. et al. (2009) FASEB J. 23:241.
  2. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
  3. Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.
  4. Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.
  5. Su, H. et al. (2013) Basic Res. Cardiol. 108:315.
  6. Sun, Y. et al. (2013) Circulation 128:S113.
  7. Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305:R522.
  8. Hwang, Y.C. et al. (2013) Int. J. Obes. (Lond) [Epub ahead of print].
Long Name
C1q and Tumor Necrosis Factor Related Protein 9
Entrez Gene IDs
338872 (Human); 239126 (Mouse)
Alternate Names
AQL1; C1q and tumor necrosis factor related protein 9; C1qTNF9; C1QTNF9AComplement C1q tumor necrosis factor-related protein 9; complement C1q tumor necrosis factor-related protein 9A; CTRP9; MGC48915

Citation for Recombinant Human CTRP9/C1qTNF9 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. C1q/TNF-related protein 9 inhibits the cholesterol-induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating AMP-dependent kinase
    Authors: Q Liu, H Zhang, J Lin, R Zhang, S Chen, W Liu, M Sun, W Du, J Hou, B Yu
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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