Recombinant Human CTRP9/C1qTNF9 Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce CXCL10 secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 1.5-7.5 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Asn16-Pro333, with an N-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    His
  • Predicted Molecular Mass
    34 kDa
  • SDS-PAGE
    36-40 kDa, reducing conditions
6537-TN
 
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human CTRP9/C1qTNF9 stimulates CXCL10 secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 1.5-7.5 μg/mL.
Background: CTRP9/C1qTNF9
C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Human C1qTNF9 shares 85% amino acid sequence identity with the mouse and rat orthologs. Both the mouse and human C1qTNF9 proteins are expressed in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). They have both also been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet‑induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).
  • References:
    1. Wong, G.W. et al. (2009) FASEB J. 23:241.
    2. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
    3. Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.
    4. Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.
    5. Su, H. et al. (2013) Basic Res. Cardiol. 108:315.
    6. Sun, Y. et al. (2013) Circulation 128:S113.
    7. Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305:R522.
    8. Hwang, Y.C. et al. (2013) Int. J. Obes. (Lond) [Epub ahead of print].
  • Long Name:
    C1q and Tumor Necrosis Factor Related Protein 9
  • Entrez Gene IDs:
    338872 (Human); 239126 (Mouse)
  • Alternate Names:
    AQL1; C1q and tumor necrosis factor related protein 9; C1qTNF9; C1QTNF9AComplement C1q tumor necrosis factor-related protein 9; complement C1q tumor necrosis factor-related protein 9A; CTRP9; MGC48915
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. C1q/TNF-related protein 9 inhibits the cholesterol-induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating AMP-dependent kinase
    Authors: Q Liu, H Zhang, J Lin, R Zhang, S Chen, W Liu, M Sun, W Du, J Hou, B Yu
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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