Recombinant Human CUL3/RBX2 Complex His-tag Protein, CF Summary
Met1 - Ala768 with a N-terminal 10-His tag (CUL3), Met1 - Lys113 (RBX2)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: CUL3/RBX2 Complex
Cullin-3 (CUL3) is a core component of multiple BCR (BTB-CUL3-RBX) E3 Ubiquitin ligase complexes that mediate the ubiquitination of several classes of signaling and structural proteins. In the BCR complex, CUL3 serves as a scaffold that organizes one or more BTB (BR-C, Ttk and Bab, also known as a POZ domain) substrate recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. Substrate specificity of a BCR ligase is determined by the BTB domain protein(s) associated with the ligase, though BTB-independent CUL3 ligase activity has been reported. In vivo, the E3 ubiquitin ligase of the BCR complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.
- Baek K., et al. (2020) Nature 578: 461.
- Choo Y.Y & Hagen T. (2012) PLoS One 7: e41350.
- Davidge B., et al. (2019) J. Cell Sci. 132: jcs233049.
- Duda D.M., et al. (2012) Mol. Cell 47: 371.
- Stogios P.J. et al. (2005) Genome Biol. 6: R82.
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