Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

R&D Systems | Catalog # E3-651

His10 Neddylated
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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein (E3-651)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Source

Sf 21 (baculovirus)

Accession Number

Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1), Q96SW2.1 (CRBN)

Applications

Bioactivity
View all CUL4A/RBX1/DDB1/CRBN Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/RBX1/DDB1/CRBN Complex protein
This complex consists of an N-terminal 10-His tagged, neddylated Cullin-4A, untagged RBX1, untagged DDB1, and untagged CRBN.

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

104 kDa (Neddylated CUL4A), 12 kDa (RBX1), 127 kDa (DDB1), 51 kDa (CRBN)

Activity

Typical concentration to support in vitro conjugation reactions will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-651
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL4A/RBX1/DDB1/CRBN Complex

Cullin-4A is a core component of multiple cullin-RING type ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-Cul4-x-box), Cullin-4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the Rbx1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on the neddylation of the cullin subunit, though neddylation may be dispensable in some in vitro reactions.  

References

  1. Fischer, E.S., et al.  (2014) Nature  512:49.
  2. He, Y.J., et al.  (2006) Genes Dev.  20:2949.
  3. Ito, T., et al.  (2010) Science  327:1345.
  4. Wang, H., et al. (2006) Mol. Cell  22:383.

Long Name

Cullin-4A/RING Box Protein 1/Damage Specific DNA Binding Protein 1/Cereblon Complex

UniProt

Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1), Q96SW2.1 (CRBN)

Additional CUL4A/RBX1/DDB1/CRBN Complex Products

Product Documents for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

For research use only

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FAQs for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

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  • Q: What is the main difference between the Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF (Catalog # E3-651) and the Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF (Catalog # E3-650)?

    A: The main difference between these products is that Catalog # E3-651 includes NEDD8 in the complex with CUL4A, RBX1, DDB1, and CRBN. CUL4A is neddylated, or attached to NEDD8. Catalog # E3-650 contains all of the same proteins in a complex (except NEDD8), but CUL4A is not neddylated.

    For this complex, neddylation is required for ligase activity in vivo, but generally not for in vitro activity. For in vitro applications, both complexes should work similarly and Catalog # E3-651 should be able to perform all of the functions of Catalog # E3-650.

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