Recombinant Human CXCL10/IP-10 Protein, CF

(36 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED50 for this effect is 0.03‑0.18 µg/mL.
  • Source
    E. coli-derived Val22-Pro98, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    8.7 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
266-IP/CF
 
266-IP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human CXCL10/IP-10/CRG-2 (Catalog # 266-IP/CF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3. The ED50 for this effect is 0.03-0.18 μg/mL.
1 µg/lane of Recombinant Human CXCL10/IP-10/CRG-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.
Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF‑ alpha, IL-12 and viruses. Additional cell types that have been shown to express CXCL10 include activated T‑lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T‑lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes.

  • References:
    1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
    2. Wang, X. et al.(1996) J. Biol. Chem. 271:24286.
  • Entrez Gene IDs:
    3627 (Human); 15945 (Mouse)
  • Alternate Names:
    C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence
    Authors: N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra
    Nat Commun, 2017;8(0):15380.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells
    Authors: H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt
    Oncol Lett, 2017;13(6):4224-4230.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Islet inflammation and ductal proliferation may be linked to increased pancreatitis risk in type 2 diabetes
    Authors: B Schludi, ASM Moin, C Montemurro, T Gurlo, AV Matveyenko, D Kirakossia, DW Dawson, SM Dry, PC Butler, AE Butler
    JCI Insight, 2017;2(13):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies
    Mucosal Immunol, 2016;9(6):1584-1595.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
    Authors: Lo H, Lai T, Li C, Wu W
    Acta Pharmacol Sin, 2014;35(3):339-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014;133(4):1227-30.
    Species: Human
    Sample Type:
  7. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  8. Human trophectoderm apposition is regulated by interferon gamma-induced protein 10 (IP-10) during early implantation.
    Authors: Sela H, Goldman-Wohl D, Haimov-Kochman R, Greenfield C, Natanson-Yaron S, Hamani Y, Revel A, Lavy Y, Singer O, Yachimovich-Cohen N, Turetsky T, Mandelboim O, Reubinoff B, Yagel S
    Placenta, 2013;34(3):222-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Profibrotic activities for matrix metalloproteinase-8 during bleomycin-mediated lung injury.
    Authors: Craig V, Quintero P, Fyfe S, Patel A, Knolle M, Kobzik L, Owen C
    J Immunol, 2013;190(8):4283-96.
    Species: Mouse
    Sample Type: Protein
    Application: EnzAct
  10. Adenovirus-engineered human dendritic cells induce natural killer cell chemotaxis via CXCL8/IL-8 and CXCL10/IP-10.
    Authors: Vujanovic, Lazar, Ballard, Wenners, Thorne, Stephen, Vujanovic, Nikola L, Butterfield, Lisa H
    Oncoimmunology, 2012;1(4):448-457.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Chemokine receptor CXCR3 promotes growth of glioma.
    Authors: Liu C, Luo D, Reynolds BA
    Carcinogenesis, 2011;32(2):129-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Type I interferon signaling in hematopoietic cells is required for survival in mouse polymicrobial sepsis by regulating CXCL10.
    Authors: Kelly-Scumpia KM, Scumpia PO, Delano MJ, Weinstein JS, Cuenca AG, Wynn JL, Moldawer LL
    J. Exp. Med., 2010;207(2):319-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009;1(4):4ra10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. CXCR3 activation by lentivirus infection suppresses neuronal autophagy: neuroprotective effects of antiretroviral therapy.
    Authors: Zhu Y, Vergote D, Pardo C, Noorbakhsh F, McArthur JC, Hollenberg MD, Overall CM, Power C
    FASEB J., 2009;23(9):2928-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Potential role of chemerin in recruitment of plasmacytoid dendritic cells to diseased skin.
    Authors: Skrzeczynska-Moncznik J, Wawro K, Stefanska A, Oleszycka E, Kulig P, Zabel BA, Sulkowski M, Kapinska-Mrowiecka M, Czubak-Macugowska M, Butcher EC, Cichy J
    Biochem. Biophys. Res. Commun., 2009;380(2):323-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  20. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  21. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  22. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007;82(4):946-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  24. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  25. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007;179(7):4840-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  26. Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
    Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
    Exp. Cell Res., 2006;312(10):1909-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
  28. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  29. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: Virus
    Sample Type: Virus
    Application: Binding Assay
  30. Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: relevance for the development of human breast cancer.
    Authors: Datta D, Flaxenburg JA, Laxmanan S, Geehan C, Grimm M, Waaga-Gasser AM, Briscoe DM, Pal S
    Cancer Res., 2006;66(19):9509-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
    Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
    Arthritis Rheum., 2005;52(5):1504-16.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  33. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus.
    Authors: Wald O, Pappo O, Safadi R, Dagan-Berger M, Beider K, Wald H, Franitza S, Weiss I, Avniel S, Boaz P, Hanna J, Zamir G, Eid A, Mandelboim O, Spengler U, Galun E, Peled A
    Eur. J. Immunol., 2004;34(4):1164-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  35. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  36. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001;31(4):1037-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 36 Citations

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