Recombinant Human CXCL5/ENA-70 Protein

R&D Systems | Catalog # 649-EN

Soon to be discontiued.
R&D Systems
Discontinued Product
649-EN has been discontinued. View all CXCL5/ENA-70 products.

Key Product Details

Source

E. coli

Accession Number

P42830

Applications

Bioactivity
View all CXCL5/ENA-70 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CXCL5/ENA-70 protein
Arg45-Asn114

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg45

Predicted Molecular Mass

7.7 kDa

Activity

Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474.
The ED50 for this effect is 0.1‑0.3 µg/mL.

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2.
The ED50 for this effect is 0.15-0.75 ng/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 649-EN in the following applications:

Formulation, Preparation, and Storage

649-EN
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL5/ENA-70

CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR- CXC chemokines which are angiostatic (1 - 3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and 35% - 51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation upregulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3 - 6). Its upregulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6 - 9). Full length CXCL5 (78 aa) is trimmed at the N‑terminal end by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds DARC, a decoy chemokine receptor which can limit CXCR2-mediated responses (13, 14).

References

  1. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  2. Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250.
  3. Walz, A.. et al. (1991) J. Exp. Med. 174:1355.
  4. Strieter, R.M. et al. (1992) Immunol. Invest. 21:549.
  5. Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012.
  6. Begley, L.A. et al. (2008) Neoplasia 10:244.
  7. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
  8. Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465.
  9. Miyazaki, H. et al. (2006) Cancer Res. 66:4279.
  10. Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
  11. Nufer, O. et al. (1999) Biochemistry 38:636.
  12. Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
  13. Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219.
  14. Horton, L.W. et al. (2007) Cancer Res. 67:9791.

Alternate Names

ENA-70, ENA70

Entrez Gene IDs

6374 (Human)

Gene Symbol

CXCL5

UniProt

P42830

Product Documents for Recombinant Human CXCL5/ENA-70 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human CXCL5/ENA-70 Protein

For research use only

Citations for Recombinant Human CXCL5/ENA-70 Protein

Customer Reviews for Recombinant Human CXCL5/ENA-70 Protein (2)

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  • Recombinant Human CXCL5/ENA-70 Protein
    Name: shelley Cutrone
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/20/2018
  • Recombinant Human CXCL5/ENA-70 Protein
    Name: Jennifer Taggart
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/20/2018

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