Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF Summary
(3 x 104 cell/well added).
Accession # O00622
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.|
|Reconstitution||Reconstitute at 250 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, when
down-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).
- Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
- Jay, P. et al. (1997) Oncogene 14:1753.
- Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
- Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
- Leu, S.-J. et al. (2002) J. Biol. Chem. 277:46248.
- Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
- Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
- O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
- Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
- Xie, D. et al. (2004) Cancer Res. 64:1987.
- Lin, M.-T. et al. (2007) J. Biol. Chem. 282:34594.
- Monnier, Y. et al. (2008) Cancer Res. 68:7323.
- Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
- Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
- Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
- Chen, C.-C. et al. (2001) J. Biol. Chem. 276:47329.
Citations for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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CCN1 interacts with integrins to regulate intestinal stem cell proliferation and differentiation
Authors: JH Won, JS Choi, JI Jun
Nature Communications, 2022;13(1):3117.
Sample Types: Organoids
CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation
Proc Natl Acad Sci USA, 2016;113(33):E4847-56.
Sample Types: Whole Cells
Applications: Flow Cytometry
L-Proline induces a mesenchymal-like invasive program in embryonic stem cells by remodeling H3K9 and H3K36 methylation.
Authors: Comes, Stefania, Gagliardi, Miriam, Laprano, Nicola, Fico, Annalisa, Cimmino, Amelia, Palamidessi, Andrea, De Cesare, Dario, De Falco, Sandro, Angelini, Claudia, Scita, Giorgio, Patriarca, Eduardo, Matarazzo, Maria R, Minchiotti, Gabriell
Stem Cell Reports, 2013;1(4):307-21.
Sample Types: Whole Cells
Sclerostin binds and regulates the activity of cysteine-rich protein 61.
Authors: Craig TA, Bhattacharya R, Mukhopadhyay D, Kumar R
Biochem. Biophys. Res. Commun., 2010;392(1):36-40.
Sample Types: Whole Cells
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