Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

R&D Systems | Catalog # 4055-CR

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Cyr61/CCN1 Fc Chimera Protein (4055-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

O00622

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Cyr61/CCN1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Cyr61/CCN1 protein
Human Cyr61
(Ala22-Asp381)
Accession # O00622		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

66.3 kDa

SDS-PAGE

70-75 kDa, reducing conditions

Activity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271.
Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL, 100 μL/well, will mediate >25% BALB/3T3 cell adhesion
(3 x 104 cell/well added).

Reviewed Applications

Read 2 reviews rated 5 using 4055-CR in the following applications:

Formulation, Preparation, and Storage

4055-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution

Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyr61/CCN1

Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, when
down-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

References

  1. Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
  2. Jay, P. et al. (1997) Oncogene 14:1753.
  3. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
  4. Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
  5. Leu, S.-J. et al. (2002) J. Biol. Chem. 277:46248.
  6. Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
  7. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
  8. O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
  9. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
  10. Xie, D. et al. (2004) Cancer Res. 64:1987.
  11. Lin, M.-T. et al. (2007) J. Biol. Chem. 282:34594.
  12. Monnier, Y. et al. (2008) Cancer Res. 68:7323.
  13. Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
  14. Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
  15. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
  16. Chen, C.-C. et al. (2001) J. Biol. Chem. 276:47329.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Gene Symbol

CCN1

UniProt

O00622 (Human)

Additional Cyr61/CCN1 Products

Product Documents for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF (2)

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  • Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 05/20/2019
  • Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/30/2018

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