Recombinant Human DCBLD2/ESDN Protein, CF

Catalog # Availability Size / Price Qty
6269-DC-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human DCBLD2/ESDN Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation/survival of IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.7-2.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human DCBLD2/ESDN protein
Gln67-Ala528, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln67 predicted, sequencing might be blocked
Predicted Molecular Mass
51.9 kDa
SDS-PAGE
80-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6269-DC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DCBLD2/ESDN

DCBLD2 (discoidin, CUB, and LCCL domain containing protein 2), also known as CLCP1 and ESDN, is a 100-130 kDa glycoprotein that bears structural resemblance to the Neuropilin family of molecules. Mature human DCBLD2 is a type I transmembrane protein that consists of a 462 amino acid (aa) extracellular region (ECD), a 21 aa transmembrane segment, and a 226 aa cytoplasmic domain (1, 2). Within the ECD, human DCBLD2 shares approximately 88% aa sequence identity with mouse and rat DCBLD2. Alternative splicing generates an isoform that contains a 14 aa insertion in the cytoplasmic domain. DCBLD2 is expressed on vascular smooth muscle cells (VSMC), particularly in regions of arteriosclerosis and vascular injury (2, 3). It is up‑regulated on PDGF-BB stimulated VSMC and inhibits PDGF‑BB induced VSMC proliferation and migration (2‑4). It is also expressed on platelets and inhibits clot formation in a zebrafish model of thrombosis (5). Compared to normal tissue, DCBLD2 expression is down‑regulated in some gastric and neuroendocrine tumors but is up‑regulated in some metastatic lung cancers (1, 6, 7). It can be tyrosine phosphorylated in response to EGF R signaling and can inhibit colony formation and tumor cell invasiveness (6, 8, 9). Its direct association with Semaphorin 4B induces DCBLD2 degradation (10).

References
  1. Koshikawa, K. et al. (2002) Oncogene 21:2822.
  2. Kobuke, K. et al. (2001) J. Biol. Chem. 276:34105.
  3. Sadeghi, M.M. et al. (2007) Am. J. Transplant. 7:2098.
  4. Guo, X. et al. (2009) J. Biol. Chem. 284:29376.
  5. O’Connor, M.N. et al. (2009) Blood 113:4754.
  6. Kim, M. et al. (2008) Mol. Cancer Res. 6:222.
  7. Hofsli, E. et al. (2008) Br. J. Cancer 99:1330.
  8. Chen, Y. et al. (2007) Proteomics 7:2384.
  9. Orso, F. et al. (2008) FASEB J. 22:2702.
  10. Nagai, H. et al. (2007) Oncogene 26:4025.
Long Name
Discoidin, CUB and LCCL Domain Containing 2/Endothelial and Smooth Muscle Cell-derived Neuropilin-li
Entrez Gene IDs
131566 (Human); 73379 (Mouse); 155696 (Rat)
Alternate Names
CLCP1; CLCP11700055P21Rik; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; DCBLD2; discoidin, CUB and LCCL domain containing 2; discoidin, CUB and LCCL domain-containing protein 2; Endothelial and smooth muscle cell-derived neuropilin-like protein; ESDN; ESDNcoagulation factor V/VIII-homology domains protein 1

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