Recombinant Human DDB1 Protein, CF Summary
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
DNA damage-binding protein 1 (DDB1) is a component of the UV-damaged DNA binding (UV-DDB) complex which minimally consists of DDB1 and DDB2. At 1,140 amino acids in length and a predicted molecular weight of 127 kDa, human DDB1 is 99% identical to both its mouse and rat orthologues. DDB1 is also a component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin Ligase complexes where it serves to associate the Cullin-4 subunit to substrate recognition subunits such as Cereblon (CRBN), and various DCAF (DDB1-CUL4 Associate Factor) proteins. This recombinant DDB1 (UniProt Q16531) is untagged.
- Fischer E.S. et al. (2014) Nature 512: 49
- He Y.J., et al. (2006) Genes Dev. 20: 2949
- Ito T., et al. (2010) Science 327: 1345
- Wang H., et al. (2006) Mol. Cell 22: 383
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