Recombinant Human DDB1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Product Details

Recombinant Human DDB1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1 protein
Accession #
Predicted Molecular Mass
127 kDa

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: DDB1

DNA damage-binding protein 1 (DDB1) is a component of the UV-damaged DNA binding (UV-DDB) complex which minimally consists of DDB1 and DDB2.  At 1,140 amino acids in length and a predicted molecular weight of 127 kDa, human DDB1 is 99% identical to both its mouse and rat orthologues.  DDB1 is also a component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin Ligase complexes where it serves to associate the Cullin-4 subunit to substrate recognition subunits such as Cereblon (CRBN), and various DCAF (DDB1-CUL4 Associate Factor) proteins.  This recombinant DDB1 (UniProt Q16531) is untagged.

  1. Fischer E.S. et al. (2014) Nature  512: 49
  2. He Y.J., et al. (2006) Genes Dev.  20: 2949
  3. Ito T., et al. (2010) Scienc327: 1345
  4. Wang H., et al. (2006) Mol. Cell  22: 383

Long Name
Damage-specific DNA Binding Protein 1
Entrez Gene IDs
1642 (Human); 13194 (Mouse); 64470 (Rat)
Alternate Names
damage-specific DNA binding protein 1 (127kD); damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; DDB p127 Subunit; DDB1; DDBA; DNA damage-binding protein 1; DNA damage-binding protein a; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; UV-DDB1; XAP1; XAP-1; Xeroderma pigmentosum group E-complementing protein; XPCE; XPE; XPE-BF; XPE-binding factor


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