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Recombinant Human Dermatopontin Protein, CF

R&D Systems | Catalog # 4629-DP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Dermatopontin Protein (4629-DP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAH33736

Applications

Bioactivity
View all Dermatopontin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Dermatopontin protein
Gln19-Val201, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted

Predicted Molecular Mass

22.8 kDa

SDS-PAGE

23 kDa, reducing conditions

Activity

Measured by its ability to modulate collagen fibrillogenesis.
20 µg/mL of rhDermatopontin can significantly enhance the rate collagen fibrillogenesis.

Formulation, Preparation, and Storage

4629-DP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dermatopontin

Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature human Dermatopontin shares 96%, 92%, and 92% amino acid sequence identity with bovine, mouse, and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6-8). Dermatopontin is down-regulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).

References

  1. Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
  2. Superti-Furga, A. et al. (1993) Genomics 17:463.
  3. Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
  4. Cronshaw, A.D. et al. (1993) Matrix 13:255.
  5. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
  6. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
  7. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
  8. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
  9. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
  10. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
  11. Okamoto, O. et al. (1996) J. Biochem. 119:106.
  12. Okamoto, O. et al. (1999) Biochem. J. 337:537.
  13. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
  14. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.

Alternate Names

DPT, Eq1

Entrez Gene IDs

1805 (Human); 56429 (Mouse); 289178 (Rat)

Gene Symbol

DPT

UniProt

AAH33736

Additional Dermatopontin Products

Product Documents for Recombinant Human Dermatopontin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Dermatopontin Protein, CF

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Recombinant Human Dermatopontin Protein, CF

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