>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells. When 3 x 104 cells/well are added to Recombinant Human Desmocollin-1 coated plates (10 µg/mL, 100 µL/well), >45% will adhere after 90 minutes at 37 °C.
Mouse myeloma cell line, NS0-derived Arg135-Asn686, with a C-terminal 6-His tag
Formulation Lyophilized from 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Desmocollin-1 (DSC1) is a 100 kDa-110 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules (1-3). Human DSC1 is synthesized with a 105 amino acid (aa) propeptide. The mature protein consists of a 557 aa extracellular domain (ECD) that contains five cadherin-like domains, a 23 aa transmembrane segment, and a 180 aa cytoplasmic domain (4, 5). Within the ECD, human DSC1 shares 79% and 82% aa sequence identity with bovine and mouse DSC1, respectively, and 53% with human DSC2 and DSC3. An alternately spliced isoform has a substituted and truncated cytoplasmic domain (5). DSC1 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is predominantly expressed in the outer layers of stratified epithelia of the skin, tongue, and hair follicle root sheath (6, 7). It is required for both promoting epidermal differentiation and maintaining epidermal strength (8). DSC2 and DSC3, by contrast, are preferentially localized in the basal and suprabasal layers of the epidermis (1). DSC1 is not expressed in normal colon epithelium, but it is induced during colon carcinogenesis (9). Loss of DSC1 function in the skin disorders IgA pemphigus and Netherton Syndrome results from autoimmune targeting or enhanced KLK-7 mediated proteolysis, respectively (10-12).
Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
King, I.A. et al. (1993) Genomics 18:185.
Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
King, I.A. et al. (1996) J. Invest. Dermatol. 107:531.
Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
Chidgey, M. et al. (2001) J. Cell Biol. 155:821.
Khan, K. et al. (2006) Br. J. Cancer 95:1367.
Hashimoto, T. et al. (1997) J. Invest. Dermatol. 109:127.
Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
Descargues, P. et al. (2006) J. Invest. Dermatol. 126:1622.
Entrez Gene IDs:
1823 (Human); 13505 (Mouse); 291759 (Rat)
cadherin family member 1; CDHF1; desmocollin 1; Desmocollin1; Desmocollin-1; desmosomal glycoprotein 2/3; DG2/DG3 ; DG2/DG3; DSC1
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