Recombinant Human DLL3 Protein, CF

R&D Systems | Catalog # 9749-DL

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human DLL3 Protein (9749-DL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q9NYJ7.1
View all DLL3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human DLL3 protein
Human DLL3
(Ala27-Ala479)
Accession # Q9NYJ7.1		 HP GGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

49 kDa

SDS-PAGE

52-62 kDa, reducing conditions

Scientific Data Images for Recombinant Human DLL3 Protein, CF

Recombinant Human DLL3 Protein SDS-PAGE

Recombinant Human DLL3 Protein SDS-PAGE

2 μg/lane of Recombinant Human DLL3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 52-62 kDa and 40-55 kDa, respectively.

Formulation, Preparation, and Storage

9749-DL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL3

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands (1). Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain (2). Within the ECD, human DLL3 shares 86% aa sequence identity with mouse and rat DLL3. DLL3 is known as a divergent DSL ligand since it does not function in similar manner as the rest of DSL ligands. It does not activate Notch signaling through trans-activation, but autonomously attenuates signaling induced by other DSL ligands (3). A loss-of-function mutation of DLL3 is linked to axial skeletal defects in the spondylocostal dysplasia, which is linked to abnormal Notch signaling (4). DL-3 promotes proliferation and inhibits apoptosis of cancer cells. The proliferative effect mediated by DLL3 is thought to be due to increased Akt phosphorylation in cancer cells (5).

References

  1. Dunwoodie, S. L. et al. (1997) Development 124:3065.
  2. Bulman, M. P. et al. (2000) Nat. Genet. 24:438.
  3. Ladi, E. et al. (2005) J. Cell Biol. 170:983.
  4. Dunwoodie, S.L. et al. (2002) Development 129:1795.
  5. Deng S.M. et al. (2017) Biochem. Biophys. Res. Commun. 483:488.

Long Name

Delta-like 3

Alternate Names

Pudgy, SCDO1

Entrez Gene IDs

10683 (Human); 13389 (Mouse); 114125 (Rat)

Gene Symbol

DLL3

UniProt

Q9NYJ7.1

Additional DLL3 Products

Product Documents for Recombinant Human DLL3 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human DLL3 Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers
Notch Pathway

Citations for Recombinant Human DLL3 Protein, CF

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail