hh Recombinant Human DLL4 Protein 1506-D4-050: R&D Systems

Recombinant Human DLL4 Protein

(13 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 150-600 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Ser27-Pro524, with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser27
  • Predicted Molecular Mass
    55.6 kDa
  • SDS-PAGE
    60-70 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1506-D4
 
1506-D4/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG 3350 with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG 3350.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human DLL4 (Catalog # 1506‑D4) enhances BMP-2 induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 150-600 ng/mL.
1 µg/lane of Recombinant Human DLL4 was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing major bands at 60-70 kDa.
Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands result in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

  • References:
    1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
    2. Iso, Tatsuya, et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
    3. Walker, L., et al. (2001) Stem Cells 19:543.
    4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
    5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
    6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
  • Long Name:
    Delta-like 4
  • Entrez Gene IDs:
    54567 (Human); 54485 (Mouse)
  • Alternate Names:
    Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. EphB4 forward signalling mediates angiogenesis caused by CCM3/PDCD10-ablation
    Authors: C You, K Zhao, P Dammann, K Keyvani, I Kreitschma, U Sure, Y Zhu
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Selective regulation of Notch ligands during angiogenesis is mediated by vimentin
    Authors: D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  3. The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
    Authors: AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi
    Nat Commun, 2017;8(0):16002.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
    Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
    Nat Med, 2015;21(5):483-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Notch signaling plays a critical role in motility and differentiation of human first-trimester cytotrophoblasts.
    Authors: Haider S, Meinhardt G, Velicky P, Otti G, Whitley G, Fiala C, Pollheimer J, Knofler M
    Endocrinology, 2014;155(1):263-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Dll4-containing exosomes induce capillary sprout retraction in a 3D microenvironment.
    Authors: Sharghi-Namini S, Tan E, Ong L, Ge R, Asada H
    Sci Rep, 2014;4(0):4031.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Fatty acid-binding protein 4, a point of convergence for angiogenic and metabolic signaling pathways in endothelial cells.
    Authors: Harjes U, Bridges E, McIntyre A, Fielding B, Harris A
    J Biol Chem, 2014;289(33):23168-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Muller glia cells regulate Notch signaling and retinal angiogenesis via the generation of 19,20-dihydroxydocosapentaenoic acid.
    Authors: Hu J, Popp R, Fromel T, Ehling M, Awwad K, Adams R, Hammes H, Fleming I
    J Exp Med, 2014;211(2):281-95.
  9. Loss of CCM3 impairs DLL4-Notch signalling: implication in endothelial angiogenesis and in inherited cerebral cavernous malformations.
    Authors: You C, Sandalcioglu I, Dammann P, Felbor U, Sure U, Zhu Y
    J Cell Mol Med, 2013;17(3):407-18.
  10. The alarmin IL-33 is a notch target in quiescent endothelial cells.
    Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
    Am J Pathol, 2012;181(3):1099-111.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Ongoing Dll4-Notch signaling is required for T-cell homeostasis in the adult thymus.
    Authors: Billiard F, Kirshner JR, Tait M, Danave A, Taheri S, Zhang W, Waite JC, Olson K, Chen G, Coetzee S, Hylton D, Murphy AJ, Yancopoulos GD, Thurston G, Skokos D
    Eur. J. Immunol., 2011;41(8):2207-16.
    Species: Human
    Sample Type: Serum
    Application: ELISA capture
  12. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
    Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
    Genes Dev., 2008;22(2):152-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
    Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
    Cancer Res., 2008;68(6):1889-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 13 Citations
Primary Antibodies
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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