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Recombinant Human/Mouse/Rat BMP-2 Protein

R&D Systems | Catalog # 355-BM

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human/Mouse/Rat BMP-2 Protein (355-BM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P12643

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all BMP-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein
Gln283-Arg396

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

13 kDa (monomer)

SDS-PAGE

15-16 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED50 for this effect is 40-200 ng/mL.

Reviewed Applications

Read 11 reviews rated 4.8 using 355-BM in the following applications:

Scientific Data Images for Recombinant Human/Mouse/Rat BMP-2 Protein

Graph showing dose-dependent bioactivity of Human BMP-2 protein

Bioactivity of Human/Mouse/Rat BMP-2 Protein

Recombinant human/mouse/rat BMP-2 (355-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 40-200 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 355-BM
Formulation Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 355-BM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.
Reconstitution Reconstitute at 100-200 μg/mL in sterile 4 mM HCI.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).

References

  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

Long Name

Bone Morphogenetic Protein 2

Alternate Names

BDA2, BMP2, SSFSC

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Gene Symbol

BMP2

UniProt

P12643

Additional BMP-2 Products

Product Documents for Recombinant Human/Mouse/Rat BMP-2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 355-BM (with carrier)
Download SDS 355-BM/CF (carrier free)

Product Specific Notices for Recombinant Human/Mouse/Rat BMP-2 Protein

For research use only

Citations for Recombinant Human/Mouse/Rat BMP-2 Protein

Customer Reviews for Recombinant Human/Mouse/Rat BMP-2 Protein (11)

4.8 out of 5
11 Customer Ratings
5 Stars
82%
4 Stars
18%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 11 reviews Showing All
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  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 04/05/2022
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: zeji du
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 05/27/2021
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/15/2020
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/09/2019
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Immunofluorescence
    Verified Customer | Posted 10/26/2018
    Immunofluorescence for pSMAD 1,5,8 following BMP treatment
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Cody Saylor
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/07/2017
  • Name: Abby Sukarto
    Verified Customer | Posted 05/03/2017
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Javier Aragón
    Application: BMP-2 Delivery System
    Verified Customer | Posted 01/05/2017
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/13/2016
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Frank Grosso
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/15/2016
  • Recombinant Human/Mouse/Rat BMP-2 Protein
    Name: Anonymous
    Sample Tested: C2C12 mouse myoblast cell line
    Verified Customer | Posted 10/26/2015
    ALP assay measuring osteogenic induction of C2C12 cells treated with BMP-2.
    Recombinant Human/Mouse/Rat BMP-2 Protein 355-BM

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FAQs for Recombinant Human/Mouse/Rat BMP-2 Protein

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Does Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) have a histidine tag?

    A: Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) does not contain a histidine tag. The sequence of this protein contains Gln283-Arg396 aa based on Accession # P12643.

  • Q: What is the difference between the two Recombinant Human/Mouse/Rat BMP-2 Proteins (Catalog # 355-BM and 355-BEC)?

    A: Catalog # 355-BM is produced in a mammalian system using CHO cells and exhibits better activity in our routine QC bioassay, while Catalog # 355-BEC is produced in an E. coli expression system and is less expensive. Bioactivity of both Human/Mouse/Rat BMP-2 Proteins is measured in an alkaline phosphatase assay using by ATDC5 mouse chondrogenic cells. For 355-BM, the ED50 for this effect is 40-200 ng/mL, while for 355-BEC, the ED50 range is 80-480 ng/mL. We recommend reviewing our list of publications under the Citations tab for both 355-BM and 355-BEC to determine which of the two proteins is most suitable for your experiment.

  • Q: What is the sequence homology between human, mouse and rat BMP-2

    A: Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2.

  • Q: Does Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) have a histidine tag?

    A: Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) does not contain a histidine tag. The sequence of this protein contains Gln283-Arg396 aa based on Accession # P12643.

  • Q: What is the difference between the two Recombinant Human/Mouse/Rat BMP-2 Proteins (Catalog # 355-BM and 355-BEC)?

    A: Catalog # 355-BM is produced in a mammalian system using CHO cells and exhibits better activity in our routine QC bioassay, while Catalog # 355-BEC is produced in an E. coli expression system and is less expensive. Bioactivity of both Human/Mouse/Rat BMP-2 Proteins is measured in an alkaline phosphatase assay using by ATDC5 mouse chondrogenic cells. For 355-BM, the ED50 for this effect is 40-200 ng/mL, while for 355-BEC, the ED50 range is 80-480 ng/mL. We recommend reviewing our list of publications under the Citations tab for both 355-BM and 355-BEC to determine which of the two proteins is most suitable for your experiment.

  • Q: What is the sequence homology between human, mouse and rat BMP-2

    A: Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2.

  • Q: Does Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) have a histidine tag?

    A: Recombinant Human/Mouse/Rat BMP-2 Protein (Catalog # 355-BM) does not contain a histidine tag. The sequence of this protein contains Gln283-Arg396 aa based on Accession # P12643.

  • Q: What is the difference between the two Recombinant Human/Mouse/Rat BMP-2 Proteins (Catalog # 355-BM and 355-BEC)?

    A: Catalog # 355-BM is produced in a mammalian system using CHO cells and exhibits better activity in our routine QC bioassay, while Catalog # 355-BEC is produced in an E. coli expression system and is less expensive. Bioactivity of both Human/Mouse/Rat BMP-2 Proteins is measured in an alkaline phosphatase assay using by ATDC5 mouse chondrogenic cells. For 355-BM, the ED50 for this effect is 40-200 ng/mL, while for 355-BEC, the ED50 range is 80-480 ng/mL. We recommend reviewing our list of publications under the Citations tab for both 355-BM and 355-BEC to determine which of the two proteins is most suitable for your experiment.

  • Q: What is the sequence homology between human, mouse and rat BMP-2

    A: Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2.

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