DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).
Recombinant Human DPP9 Protein, CF
R&D Systems | Catalog # 5419-SE
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Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Human DPP9 Protein (5419-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Sf 21 (baculovirus)
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DPP9 protein
Arg2-Leu892 with an N-terminal Met and 6-His tag
Arg2-Leu892 with an N-terminal Met and 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No result obtained
Predicted Molecular Mass
101 kDa
SDS-PAGE
92 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC).
The specific activity is >750 pmol/min/μg, as measured under the described conditions.
The specific activity is >750 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
5419-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: DPP9
References
- Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
- Qi, S.Y. et al. (2003) Biochem. J. 373:179.
- Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
- Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
- Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
- Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.
Long Name
Dipeptidyl peptidase 9
Alternate Names
DPLP9, DPRP2
Gene Symbol
DPP9
UniProt
Additional DPP9 Products
Product Documents for Recombinant Human DPP9 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human DPP9 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human DPP9 Protein, CF
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Protocols
View specific protocols for Recombinant Human DPP9 Protein, CF (5419-SE):
Materials
- Assay Buffer: 25 mM Tris, pH 8.0
- Recombinant Human DPP9 (rhDPP9) (Catalog # 5419-SE)
- Substrate: H-Gly-Pro-AMC (Bachem, Catalog # I-1225) Prepare a 10 mM stock in DMSO.
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhDPP9 to 1 ng/µL in Assay Buffer.
- Dilute substrate to 200 µM in Assay Buffer.
- Load 50 µL of 1 ng/µL rhDPP9 into a plate, and start the reaction by adding 50 µL of 50 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
| Specific Activity (pmol/min/µg) = | Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).
Per Well:- rhDPP9: 0.050 µg
- Substrate: 100 µM