Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro‑X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The human DPP7 precursor consists of a signal peptide (aa 1‑21) and a mature chain (aa 22‑492). The purified rhDPP7 is active against Lys‑Pro-AMC and Lys‑Ala-AMC. Its activity against Lys‑Pro‑AMC is approximately 10-fold of that against Lys‑Ala‑AMC under otherwise identical conditions.
Recombinant Human DPPII/QPP/DPP7 Protein, CF
R&D Systems | Catalog # 3438-SE
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Key Product Details
- R&D Systems NS0-derived Recombinant Human DPPII/QPP/DPP7 Protein (3438-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human DPPII/QPP/DPP7 protein
Gly22-Leu492, with a C-terminal 10-His tag
Gly22-Leu492, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly22 & Arg24
Predicted Molecular Mass
54 kDa
SDS-PAGE
64 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Lys-Pro-AMC (KP-AMC).
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
3438-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: DPPII/QPP/DPP7
References
- Underwood, R. et al. (1999) J. Biol. Chem. 274:34053.
- Maes, M.B. et al. (2005) Biochem. J. 386:315.
- Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
- Lankas, G.R. et al. (2005) Diabetes 54:2988.
Long Name
Dipeptidyl-peptidase II
Alternate Names
DPP7, QPP
Gene Symbol
DPP7
UniProt
Additional DPPII/QPP/DPP7 Products
Product Documents for Recombinant Human DPPII/QPP/DPP7 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human DPPII/QPP/DPP7 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human DPPII/QPP/DPP7 Protein, CF
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Protocols
View specific protocols for Recombinant Human DPPII/QPP/DPP7 Protein, CF (3438-SE):
Materials
- Assay Buffer: 25 mM MES, pH 6.0
- Recombinant Human DPPII/QPP/DPP7 (rhDPP7) (Catalog # 3438-SE)
- Substrate Lys-Pro-AMC (Bachem, Catalog # I-1745), 10 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhDPP7 to 0.2 ng/µL in Assay Buffer.
- Dilute Substrate to 200 µM in Assay Buffer.
- Load into a black microplate 50 µL of 0.2 ng/µL rhDPP7, and start the reaction by adding 50 μL of 200 µM Substrate. Include a Substrate Blank containing Assay Buffer in place of rhDPP7 and Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891)
- rhDPP7: 0.01 µg
- Substrate: 100 µM
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