Recombinant Human E-Cadherin Fc Chimera Protein, CF

R&D Systems | Catalog # 11525-EC

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human E-Cadherin Fc Chimera Protein (11525-EC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P12830.3

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all E-Cadherin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human E-Cadherin protein
Human ECAD
(Asp 155-Ile 707)
Accession # P12830.3		 GGIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp155

Predicted Molecular Mass

87 kDa

SDS-PAGE

100-114 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells.
The ED50 for this effect is 0.100-1.50 μg/mL.

Scientific Data Images for Recombinant Human E-Cadherin Fc Chimera Protein, CF

Recombinant Human E‑Cadherin Fc Chimera Protein Bioactivity.

Recombinant Human E-Cadherin Fc Chimera Protein (Catalog # 11525-EC) supports the adhesion of the MCF-7 human breast cancer cells. The ED50 for this effect is 0.100-1.50 μg/mL.

Formulation, Preparation, and Storage

11525-EC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. 
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).

References

  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Bussemakers, M.J. et al. (1993) Mol. Biol. Rep. 17:123.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Gene Symbol

CDH1

UniProt

P12830.3

Additional E-Cadherin Products

Product Documents for Recombinant Human E-Cadherin Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human E-Cadherin Fc Chimera Protein, CF

For research use only

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail