Home / E-Cadherin / Recombinant Human E-Cadherin Protein, CF

Recombinant Human E-Cadherin Protein, CF

Catalog #: 8505-EC
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8505-EC-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human E-Cadherin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 for this effect is 0.2-1 μg/mL
Source
Human embryonic kidney cell, HEK293-derived human E-Cadherin protein
Asp155-Ile707, with a C-terminal 6-His tag
Accession #
P12830
N-terminal Sequence
Analysis
Asp155
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
61 kDa
SDS-PAGE
74-92 kDa, reducing conditions

Product Datasheets

You must select a language.

x

8505-EC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8505-EC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).

References
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Bussemakers, M.J. et al. (1993) Mol. Biol. Rep. 17:123.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Citations for Recombinant Human E-Cadherin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody
    Authors: DS Micalizzi, D Che, BT Nicholson, JF Edd, N Desai, ER Lang, M Toner, S Maheswaran, DT Ting, DA Haber
    Proceedings of the National Academy of Sciences of the United States of America, 2022;119(43):e2209563119.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot Control
  2. Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling
    Authors: M Kwak, KM Southard, WR Kim, A Lin, NH Kim, R Gopalappa, HJ Lee, M An, SH Choi, Y Jung, K Noh, J Farlow, A Georgakopo, NK Robakis, MK Kang, ML Kutys, D Seo, HH Kim, YH Kim, J Cheon, ZJ Gartner, YW Jun
    Nature Cell Biology, 2022;24(12):1739-1753.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  3. A bacterial metabolite ameliorates periodontal pathogen-induced gingival epithelial barrier disruption via GPR40 signaling
    Authors: M Yamada, N Takahashi, Y Matsuda, K Sato, M Yokoji, B Sulijaya, T Maekawa, T Ushiki, Y Mikami, M Hayatsu, Y Mizutani, S Kishino, J Ogawa, M Arita, K Tabeta, T Maeda, K Yamazaki
    Sci Rep, 2018;8(1):9008.
    Species: Bacteria
    Sample Types: Bacteria
    Applications: Bioassay
  4. Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation
    Authors: JM Caldwell, MH Collins, KA Kemme, JD Sherrill, T Wen, M Rochman, EM Stucke, L Amin, H Tai, PE Putnam, MJ Jim‚nez-Da, MR Wormald, A Porollo, JP Abonia, ME Rothenberg
    Mucosal Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01

Reviews for Recombinant Human E-Cadherin Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human E-Cadherin Protein, CF and earn rewards!

Have you used Recombinant Human E-Cadherin Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review