Recombinant Human E-Cadherin Protein, CF Summary
Asp155-Ile707, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).
- Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
- Bussemakers, M.J. et al. (1993) Mol. Biol. Rep. 17:123.
- Guillot, C. and T. Lecuit (2013) Science 340:1185.
- Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
- Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
- David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.
Citations for Recombinant Human E-Cadherin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody
Authors: DS Micalizzi, D Che, BT Nicholson, JF Edd, N Desai, ER Lang, M Toner, S Maheswaran, DT Ting, DA Haber
Proceedings of the National Academy of Sciences of the United States of America, 2022;119(43):e2209563119.
Sample Types: Cell Lysates
Applications: Western Blot Control
Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling
Authors: M Kwak, KM Southard, WR Kim, A Lin, NH Kim, R Gopalappa, HJ Lee, M An, SH Choi, Y Jung, K Noh, J Farlow, A Georgakopo, NK Robakis, MK Kang, ML Kutys, D Seo, HH Kim, YH Kim, J Cheon, ZJ Gartner, YW Jun
Nature Cell Biology, 2022;24(12):1739-1753.
Sample Types: Transfected Whole Cells
A bacterial metabolite ameliorates periodontal pathogen-induced gingival epithelial barrier disruption via GPR40 signaling
Authors: M Yamada, N Takahashi, Y Matsuda, K Sato, M Yokoji, B Sulijaya, T Maekawa, T Ushiki, Y Mikami, M Hayatsu, Y Mizutani, S Kishino, J Ogawa, M Arita, K Tabeta, T Maeda, K Yamazaki
Sci Rep, 2018;8(1):9008.
Sample Types: Bacteria
Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation
Authors: JM Caldwell, MH Collins, KA Kemme, JD Sherrill, T Wen, M Rochman, EM Stucke, L Amin, H Tai, PE Putnam, MJ Jim‚nez-Da, MR Wormald, A Porollo, JP Abonia, ME Rothenberg
Mucosal Immunol, 2017;0(0):.
Sample Types: Whole Cells
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