Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
724-ES-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (37)
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Reviews (3)

Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human E-Selectin/CD62E protein
Human E-Selectin
(Trp22 - Pro556)
Accession # P16581
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Trp22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
86 kDa (monomer)
SDS-PAGE
116 - 128 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

724-ES

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 4 weeks, -20 to -70 °C under sterile conditions after reconstitution.
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Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Citations for Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Lung-Derived Selectins Enhance Metastatic Behavior of Triple Negative Breast Cancer Cells
    Authors: SU Khan, Y Xia, D Goodale, G Schoettle, AL Allan
    Biomedicines, 2021;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Enhanced sugar chain detection by oriented immobilization of Fc-fused lectins
    Authors: M Iijima, Y Yamada, T Nakayama, S Kuroda
    Biosci. Biotechnol. Biochem., 2020;0(0):1-5.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  3. Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2
    Authors: MA Florez, KA Matatall, Y Jeong, L Ortinau, PW Shafer, AM Lynch, R Jaksik, M Kimmel, D Park, KY King
    Cell Reports, 2020;33(12):108530.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. L1CAM as an E-selectin Ligand in Colon Cancer
    Authors: FM Deschepper, R Zoppi, M Pirro, PJ Hensbergen, F Dall'Olio, M Kotsias, RA Gardner, DIR Spencer, PA Videira
    Int J Mol Sci, 2020;21(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
    Authors: EW Martin, R Malgor, VA Resto, DJ Goetz, MM Burdick
    Sci Rep, 2019;9(1):8511.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  6. Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing
    Authors: A Kelkar, Y Zhu, T Groth, G Stolfa, AB Stablewski, N Singhi, M Nemeth, S Neelamegha
    Mol. Ther., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma
    Authors: U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher
    Cancers (Basel), 2019;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Minimal dosing of leukocyte targeting TRAIL decreases triple-negative breast cancer metastasis following tumor resection
    Authors: N Jyotsana, Z Zhang, LE Himmel, F Yu, MR King
    Sci Adv, 2019;5(7):eaaw4197.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Central memory CD8+ T cells become CD69+ tissue-residents during viral skin infection independent of CD62L-mediated lymph node surveillance
    Authors: JF Osborn, SJ Hobbs, JL Mooster, TN Khan, AM Kilgore, JC Harbour, JC Nolz
    PLoS Pathog., 2019;15(3):e1007633.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  10. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
    Authors: FW Lam, Q Da, B Guillory, MA Cruz
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. C/EBP? drives interactions between human MAIT cells and endothelial cells that are important for extravasation
    Authors: CH Lee, HH Zhang, SP Singh, L Koo, J Kabat, H Tsang, TP Singh, JM Farber
    Elife, 2018;7(0):.
    Species: Human
    Sample Types:
    Applications: Bioassay
  12. P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome
    Authors: K Kitamura, K Sato, M Sawabe, M Yoshida, N Hagiwara
    Circ. J., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma
    Authors: HA Cuello, VI Segatori, M Albertó, CA Gulino, R Aschero, S Camarero, LG Mutti, K Madauss, DF Alonso, F Lubienieck, MR Gabri
    Oncotarget, 2018;9(75):34176-34188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates
    Authors: NM Cordina, N Sayyadi, LM Parker, A Everest-Da, LJ Brown, NH Packer
    Sci Rep, 2018;8(1):4521.
    Applications: Bioassay
  15. Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion
    Sci Rep, 2016;6(0):30392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
    Authors: Benjamin Malard
    Artif Organs, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Hemofiltration Procedure
  17. E-selectin Targeting PEGylated-thioaptamer Prevents Breast Cancer Metastases
    Authors: Yoshihiro Morita
    Mol Ther Nucleic Acids, 2016;5(12):e399.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  18. A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. SOS1, ARHGEF1, and DOCK2 rho-GEFs Mediate JAK-Dependent LFA-1 Activation by Chemokines
    Authors: Lara Toffali
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases
    Authors: Bo Li
    Adv Healthc Mater, 2016;0(0):.
    Applications: Bioassay
  21. Non-fucosylated CB CD34(+) cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation
    Authors: Simon N Robinson
    Cytotherapy, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis.
    Authors: Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T
    Mol Ther, 2015;23(6):1044-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  23. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015;6(0):6387.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling.
    Authors: Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K
    J Immunol, 2014;192(4):1862-9.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  25. SDA, a DNA aptamer inhibiting E- and P-selectin mediated adhesion of cancer and leukemia cells, the first and pivotal step in transendothelial migration during metastasis formation.
    Authors: Faryammanesh R, Lange T, Magbanua E, Haas S, Meyer C, Wicklein D, Schumacher U, Hahn U
    PLoS ONE, 2014;9(4):e93173.
    Species: Human
    Sample Types: Beads
    Applications: Bioassay
  26. Selectins mediate small cell lung cancer systemic metastasis.
    Authors: Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M
    PLoS ONE, 2014;9(4):e92327.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
    Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
    PLoS ONE, 2014;9(9):e107920.
    Species: Bacteria
    Sample Types: Protein
    Applications: Enzyme Assay Substrate
  28. IL-15 regulates memory CD8+ T cell O-glycan synthesis and affects trafficking.
    Authors: Nolz J, Harty J
    J Clin Invest, 2014;124(3):1013-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.
    Authors: Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D
    Mucosal Immunol, 2013;6(2):393-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Circulating tumor cells from prostate cancer patients interact with E-selectin under physiologic blood flow.
    Authors: Gakhar, Gunjan, Navarro, Vicente, Jurish, Madelyn, Lee, Guang Yu, Tagawa, Scott T, Akhtar, Naveed H, Seandel, Marco, Geng, Yue, Liu, He, Bander, Neil H, Giannakakou, Paraskev, Christos, Paul J, King, Michael, Nanus, David M
    PLoS ONE, 2013;8(12):e85143.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Survival advantages conferred to colon cancer cells by E-selectin-induced activation of the PI3K-NFkappaB survival axis downstream of Death receptor-3.
    Authors: Porquet N, Poirier A, Houle F, Pin AL, Gout S, Tremblay PL, Paquet ER, Klinck R, Auger FA, Huot J
    BMC Cancer, 2011;11(0):285.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009;20(7):1565-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  33. CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
    Authors: Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
    Cancer Res., 2008;68(3):790-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells.
    Authors: Ni&lt;/LastName&gt;&lt;ForeName&gt;Zh Z&lt;/Initial, Ni Z, Walcheck B
    Immunol. Lett., 2007;108(2):179-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006;177(7):4488-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells.
    Authors: Ni Z, Campbell JJ, Niehans G, Walcheck B
    J. Immunol., 2006;177(7):4742-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
    Authors: Takahashi R, Mizukawa Y, Yamazaki Y, Hayakawa K, Hayakawa J, Kudo A, Shiohara T
    J. Immunol., 2003;171(11):5769-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

Average Rating: 4.3 (Based on 3 Reviews)

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Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF
By JIWON LEE on 03/06/2020
Application: Immunoassay Standard

By Anonymous on 10/27/2018
Application: Binding assay/Protein-protein interaction
Reason for Rating: This product does not produce as strong a signal as 575-ES (mouse E-selectin). I am not sure if this relates to preparation or binding kinetics, however the mouse E-selectin is more broadly used in the literature, even when binding to human cell lines.

Used both mouse and human at 10 ug/mL for the flow cytometry binding assay, with Biolegend anti-human IgG-PE used as a secondary probe.


Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF
By Anonymous on 12/15/2016
Application: Binding assay/Protein-protein interaction

We treat differentiated iPSC and use this product to measure the ability of the treated cells to bind to E-Selectin.