Recombinant Human E-Selectin/CD62E His-tag Protein, CF

Catalog # Availability Size / Price Qty
10258-ES-100
Recombinant Human E-Selectin/CD62E His-tag Protein, CF Bioactivity
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Product Details
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Recombinant Human E-Selectin/CD62E His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 0.2-1 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived human E-Selectin/CD62E protein
Trp22-Pro556, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Trp22
Predicted Molecular Mass
59 kDa
SDS-PAGE
84-109 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10258-ES

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human E-Selectin/CD62E (Catalog # 10258-ES) supports the adhesion of U937 human histiocytic lymphoma cell. The ED50 for this effect is 0.2-1 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human E-Selectin/CD62E His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-109 kDa and 60-84 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

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